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JoVE Journal Bioengineering
Imagen química bioortogonal del metabolismo celular regulado por aminoácidos aromáticos
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Imagen química bioortogonal del metabolismo celular regulado por aminoácidos aromáticos

Article DOI: 10.3791/65121-v • 10:42 min • May 12th, 2023
May 12th, 2023 •
*1, *1, 1, 1, 1, 1
1Shu Chien-Gene Lay Department of Bioengineering, University of California San Diego
* These authors contributed equally

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Presentamos un protocolo para visualizar directamente las actividades metabólicas en células reguladas por aminoácidos utilizando microscopía de dispersión Raman estimulada con sonda de óxido de deuterio (agua pesada D2O) (DO-SRS), que se integra con microscopía de fluorescencia por excitación de dos fotones (2PEF).

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Bioingeniería Número 195
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