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Increasing cDNA Yields from Single-cell Quantities of mRNA in Standard Laboratory Reverse Transcriptase Reactions using Acoustic Microstreaming
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Bioengineering
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JoVE Journal Bioengineering
Increasing cDNA Yields from Single-cell Quantities of mRNA in Standard Laboratory Reverse Transcriptase Reactions using Acoustic Microstreaming

Increasing cDNA Yields from Single-cell Quantities of mRNA in Standard Laboratory Reverse Transcriptase Reactions using Acoustic Microstreaming

DOI:
10.3791/3144-v

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04:52 min

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July 11, 2011

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, , , , ,
1Florey Neuroscience Institutes and Centre for Neuroscience,University of Melbourne, 2Fluid Dynamics Group,CSIRO Materials Science and Engineering, 3Swinburne University of Technology,Faculty of Engineering and Industrial Sciences

Chapters

  • 00:05Title
  • 01:43Micromixing an RT Reaction
  • 02:59The Micromixing Advantage in RT
  • 04:10Conclusion

Summary

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We describe a novel method for increasing cDNA yield from single-cell quantities of mRNA in otherwise standard laboratory reverse transcription reactions. The novelty resides in the use of a micromixer, which utilizes the phenomenon of acoustic microstreaming, to mix fluids at microliter scales more effectively than shaking, vortexing or trituration.

Tags

CDNA YieldsSingle-cell QuantitiesMRNALaboratory Reverse Transcriptase ReactionsAcoustic MicrostreamingGene ExpressionSingle-cell LevelLabile MRNADegradationStable CDNA CopyEfficiencyStandard ReagentsMicroliter VolumesMixing ReagentsMicro-scale Mixing TechniquesMicrofluidic-based Mixing TechnologiesRT Reaction Yields

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