Bioorthogonal Imaging
Bioorthogonal Imaging - Related Videos
10:54
Preparation and Evaluation of 99mTc-labeled Tridentate Chelates for Pre-targeting Using Bioorthogonal Chemistry
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Cited by 7 •
2017
Here, we describe a protocol for radiolabeling and in vivo testing of tridentate 99mTc(I) chelate-tetrazine derivatives for pre-targeting and bioorthogonal chemistry.
06:46
Imaging Glycans in Zebrafish Embryos by Metabolic Labeling and Bioorthogonal Click Chemistry
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Cited by 11 •
2011
A click-chemistry based method that allows for the rapid, noninvasive, and robust labeling of alkyne-tagged glycans in zebrafish embryos is described. Fucosylated glycans in the enveloping layer of zebrafish embryos in the late gastrulation stage were imaged in this study.
10:42
Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids
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Cited by 9 •
2023
We present a protocol to directly visualize metabolic activities in cells regulated by amino acids using deuterium-oxide (heavy water D2O) probed stimulated Raman scattering (DO-SRS) microscopy, which is integrated with two-photon excitation fluorescence microscopy (2PEF).
10:47
Harnessing the Bioorthogonal Inverse Electron Demand Diels-Alder Cycloaddition for Pretargeted PET Imaging
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Cited by 7 •
2015
The bioorthogonal inverse electron demand Diels-Alder cycloaddition has been harnessed to create an effective and modular pretargeted PET imaging strategy for cancer. In this protocol, the steps of this methodology are described in the context of a model system employing the colorectal cancer targeted antibody huA33 and a 64Cu-labeled radioligand.
12:31
Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry
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Cited by 14 •
2012
Adenovirus particles are engineered to contain either the unnatural amino acid analogue azidohomoalanine or the azido sugar O-GlcNAz. The azide group of each is chemoselectively ligated via "click" chemistry reactions as a means of viral surface modification.
FAQs
The reporter group is introduced into the biomolecule, cell, or biological system being studied, while a complementary probe carries the detectable signal. Their chemically selective reaction links the probe to the labeled target. When the pair reacts rapidly under physiological conditions, the resulting fluorescent or otherwise detectable signal identifies the target within a complex biological environment.
Physiological conditions allow labeling to occur while cells and biological processes remain active. This matters because the signal can be collected from living systems without substantially disrupting native biochemistry. Rapid reactions also help the probe engage its introduced reporter group during dynamic events, supporting observations of changing cell behavior, protein activity, or tissue development.
Low background interference makes signal from the labeled target easier to distinguish from unrelated material in the biological environment. That contrast supports more reliable visualization of biomolecules, cells, and engineered systems. In bioengineering studies, clearer signals can help connect observed location or activity with processes such as biomaterial interactions, drug delivery, or tissue development.
A typical workflow begins by introducing a reporter group into the biomolecule, cell, or process of interest. Researchers then provide a complementary probe designed to react with that group under physiological conditions. The reaction generates a fluorescent or other detectable signal, which is used to visualize the labeled target while the system remains biologically active.
In bioengineering, the approach enables researchers to follow tissue development and monitor therapeutic systems over time. Labeling can reveal where relevant cells, biomolecules, or delivery components are located as these systems change. This time-resolved information helps researchers examine engineered tissue behavior and track drug delivery without relying only on a final endpoint measurement.
Applications include tracking cell behavior, monitoring protein activity, mapping interactions between cells or biomolecules and biomaterials, and following drug delivery. It can also support studies of tissue development. These uses connect a detectable signal to specific biological or engineered processes, allowing researchers to examine how components behave within complex living systems over time.