Cell-derived Matrix
Cell-derived Matrix - Related Videos
10:48
Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix
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Cited by 8 •
2017
Robust derivation of human induced pluripotent stem (hiPS) cells was achieved by using non-integrating Sendai virus (SeV) vector mediated reprogramming of dermal fibroblasts. hiPS cell maintenance and clonal expansion was performed using xeno-free and chemically defined culture conditions with recombinant human laminin 521 (LN-521) matrix and Essential E8 (E8) Medium.
09:40
A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix
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Cited by 42 •
2017
The extracellular matrix plays a major role in defining the microenvironment of cells and in modulating cell behavior and phenotype. We describe a rapid method for the isolation of cell-derived extracellular matrix, which can be adapted to different scales for microscopic, biochemical, proteomic, or functional studies.
11:19
Fabrication of Extracellular Matrix-derived Foams and Microcarriers as Tissue-specific Cell Culture and Delivery Platforms
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Cited by 26 •
2017
The tissue-specific extracellular matrix (ECM) is a key mediator of cell function. This article describes methods for synthesizing pure ECM-derived foams and microcarriers that are stable in culture without the need for chemical crosslinking for applications in advanced 3D in vitro cell culture models or as pro-regenerative bioscaffolds.
08:23
Fabricating a Kidney Cortex Extracellular Matrix-Derived Hydrogel
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Cited by 12 •
2018
Here we present a protocol to fabricate a kidney cortex extracellular matrix-derived hydrogel to retain the native kidney extracellular matrix (ECM) structural and biochemical composition. The fabrication process and its applications are described. Finally, a perspective on using this hydrogel to support kidney-specific cellular and tissue regeneration and bioengineering is discussed.
08:02
Fabrication of Decellularized Cartilage-derived Matrix Scaffolds
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Cited by 11 •
2019
Decellularized cartilage-derived scaffolds can be used as a scaffold to guide cartilage repair and as a means to regenerate osteochondral tissue. This paper describes the decellularization process in detail and provides suggestions to use these scaffolds in in vitro settings.
FAQs
Collagen, fibronectin, and proteoglycans contribute both physical and biochemical functions within a cell-derived matrix. Together, they help create a three-dimensional environment that supports cell attachment while presenting signals capable of influencing migration, proliferation, and differentiation. Their coordinated deposition is therefore important when researchers aim to reproduce features of a native cellular microenvironment in engineered tissues.
Preserving matrix architecture matters because the arrangement of deposited components can retain more than structural support. It can also maintain biochemical cues that cells encounter in their surroundings, allowing the scaffold to influence behavior in a context that more closely resembles native tissue. In bioengineering, this helps researchers build models with greater physiological relevance.
Controlled culture conditions provide the setting in which living cells assemble and deposit matrix components. Because the cells produce the material themselves, the resulting composition and architecture reflect the culture process rather than a preassembled structure. Managing this stage is important for generating a matrix that retains the support and signaling properties needed for later bioengineering studies.
Researchers first culture living cells under controlled conditions, allowing them to produce and deposit extracellular matrix components. Once matrix has formed, they may remove the cells while preserving the deposited architecture and signaling cues. The resulting cell-free material can then function as a biologically active scaffold for engineered tissue studies and related applications.
It is useful when a project needs a biologically active environment rather than structural support alone. Reported applications include tissue engineering, cell culture, wound repair, and regenerative medicine. In these settings, the matrix can provide a native-like context that helps regulate cell adhesion, migration, proliferation, and differentiation, making it relevant to both model development and repair-oriented research.
Researchers can examine how cells respond to the matrix through changes in adhesion, migration, proliferation, and differentiation. These behaviors provide functional evidence of how the scaffold's architecture and biochemical cues affect cell activity. Such observations are especially relevant in bioengineering because they connect the material's properties to the development of more physiologically relevant engineered tissues.