Cell Morphology
Cell Morphology - Related Videos
07:59
The Analysis of Purkinje Cell Dendritic Morphology in Organotypic Slice Cultures
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Cited by 15 •
2012
We present a protocol that permits to view and to quantitatively asses the morphology of the dendritic tree of individual Purkinje cells grown in organotypic cerebellar slice cultures. This protocol is intended to promote studies on the mechanisms of Purkinje cell dendritic development.
03:56
Bone Marrow Aspiration: A Method to Obtain Bone Marrow to Examine Cell Morphology
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2023
This video describes the technique of aspirating bone marrow from iliac spine and smear preparation for biopsy. In the example, we perform bone marrow aspiration from an acute myeloid leukemia (AML) patient and examine the cells to check for measurable residual disease (MRD), an important prognostic biomarker.
14:37
Whole-cell Patch-clamp Recordings from Morphologically- and Neurochemically-identified Hippocampal Interneurons
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Cited by 39 •
2014
Cortical networks are controlled by a small, but diverse set of inhibitory interneurons. Functional investigation of interneurons therefore requires targeted recording and rigorous identification. Described here is a combined approach involving whole-cell recordings from single or synaptically-coupled pairs of neurons with intracellular labeling, post-hoc morphological and immunocytochemical analysis.
05:57
Live-Cell Fluorescence Microscopy to Investigate Subcellular Protein Localization and Cell Morphology Changes in Bacteria
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Cited by 18 •
2019
This article provides a step-by-step guide to investigate protein subcellular localization dynamics and to monitor morphological changes using high-resolution fluorescence microscopy in Bacillus subtilis and Staphylococcus aureus.
11:41
Automated Sholl Analysis of Digitized Neuronal Morphology at Multiple Scales
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Cited by 61 •
2010
We have developed a computer program to analyze neuronal morphology. In combination with two existing open source analysis tools, our program performs Sholl analysis and determines the number of neurites, branch points, and neurite tips. The analyses are performed so that local changes in neurite morphology can be observed.
FAQs
Environmental cues can change cell spreading, alignment, adhesion, and spatial organization. Substrate properties, mechanical forces, and biochemical signals influence how cells arrange themselves and interact with their surroundings. In bioengineering, these morphological responses help reveal whether a designed microenvironment is directing cellular behavior in the intended way, even before researchers interpret molecular or functional measurements.
These features provide distinct evidence about how cells interact with an engineered environment. Spreading reflects the extent of cellular contact with a surface, alignment reveals coordinated orientation, and adhesion indicates attachment to the surrounding material. Tracking their changes helps researchers evaluate how substrate properties and other cues influence cell behavior in biomaterials, engineered tissues, and related systems.
Morphological measurements offer structural evidence that complements molecular and functional assays. A change in cell shape or organization may indicate a response to an engineered microenvironment, while molecular and functional tests provide additional information about cellular state and performance. Considering these forms of evidence together can clarify how design conditions regulate behavior rather than relying on morphology alone.
Spatial organization can indicate whether cells are arranging themselves in patterns associated with developing or engineered tissue structure. Changes in organization, alignment, and adhesion may help researchers evaluate tissue maturation and the influence of the surrounding environment. This makes cell morphology useful for comparing engineered tissue conditions and assessing whether a bioengineered construct is developing as intended.
Researchers commonly assess cell morphology through microscopy followed by image analysis. These approaches allow them to examine features such as cell size, shape, spreading, alignment, adhesion, and organization. The resulting measurements can be compared across engineered environments or conditions, providing a structured way to evaluate cellular responses in biomaterials, tissues, and other bioengineering systems.
Morphological measurements support evaluation across several bioengineering platforms, including engineered tissues, biomaterials, organ-on-chip systems, and cell-based therapies. In each setting, changes in cellular appearance and organization can provide evidence of how the designed environment affects cells. Such measurements help researchers assess material or system performance and guide improvements in regenerative and diagnostic technologies.
Cell morphology can help compare cellular responses under disease-related conditions or during development of cell-based therapies. Researchers may examine changes in shape, spreading, adhesion, alignment, or organization to identify differences between environments or treatment contexts. Interpreted alongside molecular and functional assays, these observations can support evaluation of engineered therapies and diagnostic technologies.