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JoVE Journal Neuroscience
The Analysis of Purkinje Cell Dendritic Morphology in Organotypic Slice Cultures
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The Analysis of Purkinje Cell Dendritic Morphology in Organotypic Slice Cultures

Article DOI: 10.3791/3637 • 08:00 min • March 21st, 2012
March 21st, 2012 •
1, 1
1Anatomical Institute, Department of Biomedicine, University of Basel

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We present a protocol that permits to view and to quantitatively asses the morphology of the dendritic tree of individual Purkinje cells grown in organotypic cerebellar slice cultures. This protocol is intended to promote studies on the mechanisms of Purkinje cell dendritic development.

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Purkinje Cell Dendritic Morphology Organotypic Slice Cultures Sagittal Plane Postnatal Period Anti-Calbindin D28K EGFP Jackson Labs Cerebellar Slice Cultures Dendritic Development Dendritic Expansion Quantitative Evaluation Dendritic Tree Size Branch Point Numbers Anti-calbindin Stained Protein Kinase C (PKC) Acti
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