Flow Cell Technique
Flow Cell Technique - Related Videos
10:43
Flow Cytometry Purification of Mouse Meiotic Cells
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Cited by 65 •
2011
An efficient method to obtain highly purified viable meiotic fractions from mouse testis is described, which combines a refined cell dissociation protocol with fluorescent activated cell sorting (FACS). This method takes advantage of differences in the DNA content and nuclear density of discrete meiotic fractions.
19:26
Measuring Intracellular Ca2+ Changes in Human Sperm using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging
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Cited by 30 •
2013
Intracellular Ca2+ dynamics are very important in sperm physiology and Ca2+-sensitive fluorescent dyes constitute a versatile tool to study them. Population experiments (fluorometry and stopped flow fluorometry) and single cell experiments (flow cytometry and single cell imaging) are used to track spatio-temporal [Ca2+] changes in human sperm cells.
09:39
Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry
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Cited by 31 •
2015
Many different methods exist for the measurement of extracellular vesicles (EVs) using flow cytometry (FCM). Several aspects should be considered when determining the most appropriate method to use. Two protocols for measuring EVs are presented, using either individual detection or a bead-based approach.
03:40
In Vitro Photoacoustic Flow Cytometry: A Non-invasive Flow Photoacoustic Technique to Detect Nanoparticle-bearing Circulating Ovarian Cancer Cells
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2023
In this video, we present a photoacoustic flow cytometric detection of ovarian cancer cells bearing nanoparticles in a circulating in vitro flow chamber. This non-invasive technique successfully identifies circulating tumor cells in a flow system utilizing a contrast agent.
07:34
Schlieren Imaging: A Technique to Visualize Supersonic Flow Features
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2023
Source: Jose Roberto Moreto, Jaime Dorado, and Xiaofeng Liu, Department of Aerospace Engineering, San Diego State University, San Diego, CA Military jet fighters and projectiles can fly at incredible speeds that exceed the speed of sound, which means they are traveling at a supersonic speed. The speed of sound is the speed at which a sound wave propagates through a medium, which is 343 m/s. Mach numbers are used to gauge the flight speed of an object relative to the speed of sound. An object...
FAQs
Shear stress depends mainly on the imposed flow rate, channel geometry, and fluid properties. The pump establishes how quickly liquid moves, while the chamber design shapes the flow environment. Fluid characteristics also affect the mechanical forces transmitted to cells, biomaterials, or tissue constructs. Controlling these variables allows researchers to relate defined fluid conditions to biological or material responses.
Pump settings regulate perfusion through the chamber, whereas channel geometry determines how that flow is distributed around the tested material. Together, they influence shear stress, nutrient delivery, and exposure to test compounds. A defined chamber therefore helps researchers create reproducible mechanical and transport conditions instead of relying on uncontrolled fluid movement across a biological sample.
Flow affects how nutrients and test compounds reach cells, biomaterials, and tissue constructs. The resulting mass transport can change the availability of substances at the sample surface and connect fluid conditions with biological outcomes. By controlling perfusion, researchers can examine responses under defined delivery conditions, which is useful when evaluating cell behavior or tissue-engineering environments.
A typical setup places the biological material or biomaterial within a defined chamber, connects the chamber to a pump, and establishes a controlled liquid flow. Researchers then select conditions based on the desired flow rate, channel geometry, and fluid properties. The resulting perfusion exposes the sample to defined shear and transport conditions for subsequent study of its response.
This approach is useful when investigators need to study cells, biomaterials, or tissue constructs under controlled perfusion rather than in an undefined fluid environment. Applications described for bioengineering include examining cell behavior, evaluating biomaterial performance, supporting tissue-engineering studies, and investigating how microfluidic device designs shape fluid and biological interactions.
Results can link controlled mechanical conditions, such as fluid shear, with biological or material behavior. Researchers may use the system to assess how cells respond to perfusion, how biomaterials perform during liquid exposure, or how tissue constructs receive nutrients or test compounds. These observations also inform the design of microfluidic devices and other bioengineering systems.