Journal
/
Biology
/
Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

DOI:
10.3791/52484-v

•

09:39 min

•

March 17, 2015

•

, ,
1Blood Systems Research Institute, 2Department of Medicine,University of California, San Francisco, 3Department of Laboratory Medicine,University of California, San Francisco

Chapters

  • 00:05Title
  • 01:23Individual Extracellular Vesicle Detection
  • 05:33Bead-based Detection of EVs and Exosomes
  • 07:19Results: Representative Individual and Bead-based Detection Data
  • 08:49Conclusion

Summary

Automatic Translation

Automatic Translation

Many different methods exist for the measurement of extracellular vesicles (EVs) using flow cytometry (FCM). Several aspects should be considered when determining the most appropriate method to use. Two protocols for measuring EVs are presented, using either individual detection or a bead-based approach.

Tags

Keywords: Extracellular VesiclesFlow CytometryCell-cell CommunicationBiological ProcessesAnalysis TechniquesEV ProcessingBead-based ApproachIndividual Detection MethodSignal-to-noise RatioBackground FluorescenceClinical SamplesExosomes

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/52484?article_type=v

Flow Cytometry Purification of Mouse Meiotic Cells
check_url/52484?article_type=v

Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes
check_url/52484?article_type=v

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization
check_url/52484?article_type=v

Sample Preparation for Mass Cytometry Analysis
check_url/52484?article_type=v

LED Thermo Flow — Combining Optogenetics with Flow Cytometry
check_url/52484?article_type=v

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry
check_url/52484?article_type=v

Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media
check_url/52484?article_type=v

Purification and Analysis of Caenorhabditis elegans Extracellular Vesicles
check_url/52484?article_type=v

Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles
check_url/52484?article_type=v

Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues

Read Article