Mononucleated Cells
Mononucleated Cells - Related Videos
06:09
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Cited by 37 •
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Immature dendritic cells can be selectively differentiated into tolerogenic or mature dendritic cells to regulate the balance between immunity and tolerance. This work presents a means to generate from immature monocyte derived dendritic cells (moDCs), in vitro tolerogenic and mature moDCs that differ in metabolic phenotypes.
10:04
Tractable In Vivo Reprogramming of Tumor Cells to Type 1 Conventional Dendritic Cell-like Cells
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22:06
Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
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Cited by 7 •
2007
This protocol details the derivation of transplantable hematopoietic stem cells from mouse embryonic stem cells (ESC) and their subsequent injection into lethally irradiated recipient mice. Briefly, ESC are differentiated as embryoid bodies, which are then infected with retroviral HoxB4 and co-cultured with OP9 stromal cells and hematopoietic cytokines.
02:47
Human ES cells: Starting Culture from Frozen Cells
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2006
Here we demonstrate how our lab begins a HuES human embryonic stem cell line culture from a frozen stock.
10:54
Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations
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Cited by 2 •
2012
Metaphase to anaphase transition is triggered through anaphase-promoting complex (APC/C)-dependent ubiquitination and subsequent destruction of cyclin B. Here, we established a system which, following pulse-chase labeling, allows monitoring cyclin B proteolysis in entire cell populations and facilitates the detection of interference by the mitotic checkpoint.
FAQs
During centrifugation, blood components distribute through the density gradient according to their buoyant density. This arrangement creates distinct regions within the sample, allowing the mononuclear fraction to be identified and collected at the gradient interface. The approach concentrates the relevant cells from whole blood for downstream biomedical research and bioengineering studies.
The collected fraction includes lymphocytes and monocytes, two cell populations associated with immune function. Their presence gives the sample functional diversity rather than representing a single uniform cell type. Consequently, isolated mononucleated cells can support investigations of immune responses and provide biologically relevant inputs for biomaterial and tissue-engineering research.
The gradient interface serves as the collection location for the mononuclear fraction after centrifugation has distributed blood components by buoyant density. Locating and recovering this interface enables researchers to obtain the cell population of interest instead of working with whole blood. This focused fraction is useful when experimental analyses require access to lymphocytes and monocytes.
Isolated mononucleated cells provide a biological system for evaluating how biomaterials relate to immune responses. Because the fraction contains lymphocytes and monocytes, researchers can examine material-associated biological effects using cells with functional diversity. This application connects blood-cell processing with biomaterial assessment and helps place material studies in a biologically relevant research context.
A basic workflow begins with whole blood, applies density-gradient centrifugation, and then identifies the mononuclear fraction at the gradient interface. Researchers collect that interface for subsequent study or use. The procedure depends on separation by buoyant density, so careful recovery of the indicated fraction is central to obtaining the intended cell population.
Researchers may use isolated cells to study immune responses, evaluate biomaterials, model disease, or develop cell-based therapies. The same preparation also supports work in tissue engineering, diagnostics, and regenerative medicine. Its value comes from combining accessibility with functional diversity, allowing one cell source to contribute to several biomedical and bioengineering applications.
The cells can provide experimental material for examining immune behavior, testing biomaterial interactions, modeling disease-related processes, and informing cell-based therapy development. In tissue engineering and regenerative medicine, they can contribute to studies involving cellular responses and therapeutic design. In diagnostics, their availability supports investigation of biologically relevant cellular characteristics.