Optical Probe Construction
Optical Probe Construction - Related Videos
09:35
Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents
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Cited by 14 •
2012
Biosensors interface with complex, biological environments and perform targeted detection by combining highly sensitive sensors with highly specific probes attached to the sensor via surface modification. Here, we demonstrate the surface functionalization of silica optical sensors with biotin using silane coupling agents to bridge the sensor and the biological environment.
11:21
Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells
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Cited by 5 •
2010
This protocol describes a general approach to perform photoconversion of fluorescent proteins on a confocal laser scanning microscope. We describe procedures for the photoconversion of puried protein samples, as well as for dual-probe optical highlighting in live cells with mOrange2 and Dronpa.
09:12
Construction of a High Resolution Microscope with Conventional and Holographic Optical Trapping Capabilities
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Cited by 4 •
2013
The system described herein employs a traditional optical trap as well as an independent holographic optical trapping line, capable of creating and manipulating multiple traps. This allows for the creation of complex geometric arrangements of refractive particles while also permitting simultaneous high-speed, high-resolution measurements of the activity of biological enzymes.
13:58
Probing C84-embedded Si Substrate Using Scanning Probe Microscopy and Molecular Dynamics
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Cited by 1 •
2016
This paper reports the nanomaterial fabrication of a fullerene Si substrate inspected and verified by nanomeasurements and molecular dynamic simulation.
09:39
Optical Biosensing
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2023
Optical biosensors utilize light to detect the binding of a target molecule. These sensors can utilize a label molecule, which produces a measurable signal such as fluorescence, or these sensors can be label-free and use the changes in optical properties, such as refractive index, to sense for the binding of the target molecule. This video introduces both label and label-free optical biosensors, demonstrates their use in the laboratory, and shows some applications of the technology.
FAQs
The biological event determines which optical property changes: fluorescence, absorption, or emitted light. Binding can alter the reporter through the recognition element, while enzymatic activity or a changed local chemical environment can produce a measurable shift. This coupling lets investigators translate molecular interactions into signals that can be measured and compared in bioengineering experiments.
Each component contributes a distinct design function. The optical reporter supplies the measurable light response, the recognition element directs interaction with the intended biological target, and the scaffold or linker organizes their relationship. Changing how these parts are combined can influence whether target binding, enzymatic activity, or local chemistry produces a detectable signal.
Selectivity, stability, and signal intensity are connected but separate design concerns. Selectivity determines how specifically the probe responds to the target, stability supports its performance under the intended conditions, and signal intensity affects how clearly the event can be measured. Balancing these properties helps tailor a probe to a particular detection or imaging task.
Construction begins by specifying the biological target or event and selecting an optical reporter, recognition element, and scaffold or linker that suit that goal. The components are then assembled into a probe design. Researchers tailor the resulting structure and performance according to the desired selectivity, stability, and signal intensity for the intended biological measurement.
Applications extend across biosensing, cellular imaging, disease-marker detection, and monitoring of biochemical processes. The appropriate design depends on whether the goal is targeted detection, visualization in cells, recognition of a disease-associated marker, or tracking molecular activity. These use cases show why probe construction must be matched to the biological question and desired light-based measurement.
In bioengineering, the main value lies in connecting molecular activity with quantitative biological measurements. A probe can be designed for targeted detection, cellular imaging, disease-marker detection, or monitoring of biochemical processes. Matching the reporter and recognition architecture to the biological question helps translate a specific event into a light-based readout suited to that application.