Pump Probe Microscopy
Pump Probe Microscopy - Related Videos
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Probing C84-embedded Si Substrate Using Scanning Probe Microscopy and Molecular Dynamics
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This paper reports the nanomaterial fabrication of a fullerene Si substrate inspected and verified by nanomeasurements and molecular dynamic simulation.
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Surface Potential Measurement of Bacteria Using Kelvin Probe Force Microscopy
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Here, we present a protocol explaining the use of Kelvin probe force microscopy as a tool for generating high resolution nano-scale surface potential maps. This tool was applied to assess the role of surface potential on the binding capacity of microorganisms to substrate surfaces.
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ATP Driven Pumps III: V-type Pumps
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V-type pumps are ATP-driven pumps found in the vacuolar membranes of plants, yeast, endosomal and lysosomal membranes of animal cells, plasma membranes of a few specialized eukaryotic cells, and some prokaryotes. They are also known as the V1Vo-ATPase, that couple ATP hydrolysis to transport protons against a concentration gradient. The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
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An Experimental Protocol for Femtosecond NIR/UV - XUV Pump-Probe Experiments with Free-Electron Lasers
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01:34
ATP Driven Pumps II: P-type Pumps
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2023
The P-type pumps are a large family of integral membrane transporter ATPases. They are divided into five major types based on substrate specificity, from I to V. A typical P-type pump has three cytosolic domains: nucleotide-binding (N), phosphorylation (P), and activator (A) domains. These domains are connected to the membrane-spanning helices by short amino acid segments. ATP hydrolysis and covalent phosphoenzyme intermediate formation are crucial parts of the catalytic cycle. At the highly...
FAQs
The delay between pump and probe pulses acts as a controllable time coordinate. By changing that delay and recording the resulting optical response, researchers can follow how an excited state evolves rather than capturing only a single moment. This makes Pump Probe Microscopy useful for separating ultrafast molecular or electronic changes from slower responses that persist into the nanosecond range.
Absorption changes and refractive-index changes provide complementary readouts of excitation-induced behavior. A change in absorption indicates that the sample’s interaction with light has shifted, whereas a refractive-index change reports altered optical propagation through the material. Recording these signals over delay helps distinguish response characteristics among pigments, nanoparticles, biomaterials, and cellular components.
Fluorescence is not the only source of contrast in this approach. Pump Probe Microscopy can monitor excitation-related changes in absorption or refractive index, allowing characterization of structures that may not provide a useful fluorescent signal. This expands optical analysis to nonfluorescent pigments, engineered materials, nanoparticles, and cellular components that fluorescence-focused measurements might not fully represent.
A basic measurement directs a brief pump pulse onto the sample, applies a probe pulse after a controlled delay, and records the resulting optical change. The delay is then varied across the desired time range, from femtoseconds to nanoseconds, to build a temporal response. Researchers can compare these response traces across different materials or sample regions.
Within bioengineering, the method can be applied to pigments, nanoparticles, biomaterials, and cellular components, including structures that are not readily assessed through fluorescence alone. These targets connect optical dynamics with engineered material behavior and biological context. Measurements can therefore support the study of how material composition or cellular location relates to excitation-driven optical responses.
Time-resolved optical measurements can help assess energy transfer and photothermal responses after excitation. In bioengineering, those outcomes are relevant when examining how nanoparticles, pigments, or biomaterials respond to light and interact with cells. The resulting information can contribute to the development or evaluation of imaging, diagnostic, and engineered therapeutic systems without depending exclusively on fluorescence.