Arabidopsis Ovules

Arabidopsis ovules are seed-forming reproductive structures within the flower’s ovary and a widely used model for studying plant reproduction and development. Each ovule develops from a megaspore mother cell, which undergoes meiosis to produce a functional megaspore that forms the embryo sac containing the egg cell, central cell, synergids, and antipodals; after pollination, a pollen tube reaches the ovule through the micropyle and enables double fertilization. Research on Arabidopsis ovules reveals how reproductive cell identities, fertilization, seed formation, and maternal tissues are regulated, supporting studies of developmental genetics, plant fertility, crop improvement, and plant responses to environmental conditions.

Arabidopsis Ovules - Related Videos

Research

JoVE Journal - Developmental Biology

In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana

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Cited by 27 •

2017

This manuscript describes an in vitro ovule cultivation method that enables live-cell imaging of Arabidopsis zygotes and embryos. This method is utilized to visualize the intracellular dynamics during zygote polarization and the cell fate specification in developing embryos.

A Method for Characterizing Embryogenesis in Arabidopsis

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Cited by 11 •

2017

This protocol outlines a method for observing embryogenesis in Arabidopsis via ovule clearance followed by the inspection of embryo pattern formation under a microscope.

An Efficient Method for Quantitative, Single-cell Analysis of Chromatin Modification and Nuclear Architecture in Whole-mount Ovules in Arabidopsis

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Cited by 15 •

2014

We provide here an efficient and reliable protocol for immunostaining, Fluorescence in situ Hybridization, DNA staining followed by quantitative, high-resolution imaging in whole-mount Arabidopsis thaliana ovules. This method was successfully used to analyze chromatin modifications and nuclear architecture.

Microinjection of Tetrodotoxin into the Brain of an Awake Female Rat to Inhibit Ovulation

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2025

Source: Silva, C. et al. Unraveling the Role of Discrete Areas of the Rat Brain in the Regulation of Ovulation through Reversible Inactivation by Tetrodotoxin Microinjections. J. Vis. Exp. (2020)This video demonstrates the protocol for microinjection of tetrodotoxin (TTX) into the hypothalamus of a freely moving rat to transiently inhibit neuronal activity, suppress gonadotropin-releasing hormone secretion, and prevent ovulation.

Lateral Root Inducible System in Arabidopsis and Maize

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Cited by 8 •

2016

The Lateral Root Inducible System (LRIS) allows for synchronous induction of lateral roots and is presented for Arabidopsis thaliana and maize.

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