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In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana
DOI:

05:41 min

September 11, 2017

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Chapters

  • 00:05Title
  • 00:45Poly(Dimethylsoloxane) Micropillar Array Divide Preparation
  • 02:03Ovule Extraction and Time-lapse Imaging
  • 04:14Results: Representative Live-imaging of Zygote Polarization and Embryo Patterning
  • 05:03Conclusion

Summary

Automatic Translation

This manuscript describes an in vitro ovule cultivation method that enables live-cell imaging of Arabidopsis zygotes and embryos. This method is utilized to visualize the intracellular dynamics during zygote polarization and the cell fate specification in developing embryos.

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