Automated Digital Processing

Automated digital processing is the use of computer-based algorithms to convert raw digital information into organized, interpretable results with limited manual intervention. In biology, software can receive images, sensor outputs, or experimental datasets, then apply operations such as noise reduction, feature detection, image segmentation, classification, and quantitative measurement according to defined rules. These workflows help researchers analyze cells, tissues, organisms, and molecular data more rapidly and consistently than manual methods alone. By improving reproducibility, reducing processing time, and supporting large-scale analysis, automated digital processing strengthens biological imaging, laboratory automation, and data-driven research.

Automated Digital Processing - Related Videos

Research

JoVE Journal - Biology

Digital Microfluidics for Automated Proteomic Processing

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Cited by 30 •

2009

Digital Microfluidics is a technique characterized by the manipulation of discrete droplets (~nL - mL) on an array of electrodes by the application of electrical fields. It is well-suited for carrying out rapid, sequential, miniaturized automated biochemical assays. Here, we report a platform capable of automating several proteomic processing steps.

Automated Sholl Analysis of Digitized Neuronal Morphology at Multiple Scales

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Cited by 61 •

2010

We have developed a computer program to analyze neuronal morphology. In combination with two existing open source analysis tools, our program performs Sholl analysis and determines the number of neurites, branch points, and neurite tips. The analyses are performed so that local changes in neurite morphology can be observed.

Experimental Assessment of Mouse Sociability Using an Automated Image Processing Approach

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2016

This protocol describes a method to quantify mouse sociability. Mice are videotaped as they move and interact in a special cage. Movie processing allows for the automated quantification of sociability with excellent accuracy and reliability.

Automated Counterflow Centrifugal System for Small-Scale Cell Processing

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Cited by 7 •

2019

Automation is key to upscaling and cost management in cell manufacturing. This manuscript describes the use of a counterflow centrifugal cell processing device for automating the buffer exchange and cell concentration steps for small-scale bioprocessing.

Calcified Artery Preparation and Processing with Preserved Morphology and RNA for Digital Spatial Profiling

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2026

This protocol includes a step-by-step workflow for calcified vascular specimens: tissue handling, decalcification, RNA validation, calcification level detection, and region-of-interest selection strategies on the Nanostring GeoMx Digital Spatial Profiler (DSP). The goal is to present a comprehensive method for preserving vascular tissue morphology and RNA for reliable spatial transcriptomic analysis.

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