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Automated Counterflow Centrifugal System for Small-Scale Cell Processing

Article DOI: 10.3791/60423-v • 04:49 min • December 12th, 2019
December 12th, 2019 •
1,2, 3, 3, 1,2, 1, 1,2, 1,2, 3, 1,2,4
1The Ritchie Centre, Hudson Institute of Medical Research, 2Department of Obstetrics and Gynaecology, Monash University, 3Scinogy, 4Australian Regenerative Medicine Institute, Monash University

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Automation is key to upscaling and cost management in cell manufacturing. This manuscript describes the use of a counterflow centrifugal cell processing device for automating the buffer exchange and cell concentration steps for small-scale bioprocessing.

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Automated Counterflow Centrifugal System Small-scale Cell Processing Closed Automated Buffer Exchange Concentration Recovery Small Volume Automation Steps Programming Test Runs Medium Laminar Flow Head Syringe Needle Assembly Saline Solution Human Serum Albumin Culture Vessels Counting Cells Transfer Bag Luer Lock Wash Buffer Graphic User Interface Start Button Protocol Creation Valves Centrifugal Speed Pump Speed Pump Direction Action Triggers
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