Barrier Methods
Barrier Methods - Related Videos
09:23
Improved Method for the Establishment of an In Vitro Blood-Brain Barrier Model Based on Porcine Brain Endothelial Cells
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Cited by 31 •
2017
The aim of the protocol is to present an optimized procedure for the establishment of an in vitro blood-brain barrier (BBB) model based on primary porcine brain endothelial cells (pBECs). The model shows high reproducibility, high tightness, and is suitable for studies of transport and intracellular trafficking in drug discovery.
18:57
Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers
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Cited by 58 •
2013
Many therapeutic applications require safe and efficient transport of drug carriers and their cargoes across cellular barriers in the body. This article describes an adaptation of established methods to evaluate the rate and mechanism of transport of drug nanocarriers (NCs) across cellular barriers, such as the gastrointestinal (GI) epithelium.
08:40
Improved Method for the Preparation of a Human Cell-based, Contact Model of the Blood-Brain Barrier
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Cited by 16 •
2013
Establishment of human models of the blood-brain barrier (BBB) can benefit research into brain conditions associated with BBB failure. We describe here an improved technique for preparation of a contact BBB model, which permits coculturing of human astrocytes and brain endothelial cells on the opposite sides of a porous membrane.
08:48
Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants
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Cited by 15 •
2016
Existing methods for the modification of plants have limited applicability. The novel peptide-derived technology proposed here promises both simplicity and efficiency in the introduction of exogenous protein or genes into the desired intracellular compartments of intact plants.
05:48
An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity
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Cited by 7 •
2018
Here, we present a protocol to assess the blood-testis barrier integrity by injecting inulin-FITC into testes. This is an efficient in vivo method to study blood-testis barrier integrity that can be compromised by genetic and environmental elements.
FAQs
Their location determines how they obstruct sperm movement. Condoms create a sheath around the penis or line the vagina, limiting the passage of sperm, while diaphragms and cervical caps cover the cervix and block access to the uterus. This physical separation prevents sperm from completing the transport pathway needed to reach an egg.
Spermicide can be combined with some barrier methods to increase effectiveness. The barrier supplies the physical obstruction, while the spermicide adds another layer of protection against sperm reaching an egg. Because the source identifies spermicide as an optional combination rather than a feature of every barrier, its use depends on the selected method and instructions.
External and internal condoms reduce contact between sexual partners and infectious fluids by placing a material barrier around or within the relevant genital area. This can lower transmission of many sexually transmitted infections while also preventing sperm transport. The protection is not described as universal, so condoms should be understood as reducing, rather than eliminating, infection transmission.
Effectiveness depends heavily on choosing a suitable barrier and using it consistently and correctly during sexual activity. A method that is appropriate in principle may provide less reliable pregnancy prevention if it is used inconsistently or incorrectly. Thus, practical behavior and method selection are central determinants of outcomes, not merely the physical presence of a barrier.
Selection should match the available barrier options and the user’s need for a method that is used only when needed. Condoms, diaphragms, and cervical caps differ in where they are positioned, while some options may be paired with spermicide. Correct, consistent use during each relevant sexual activity supports the intended contraceptive effect.
Barrier methods provide contraception without the systemic effects associated with hormonal approaches, according to the source, and they can be stopped without requiring a continuing method. Their use is event-based rather than continuous, which makes them relevant for people seeking reversible pregnancy prevention with generally few systemic effects. Their success still depends on correct and consistent use.