Bone Core Sampling
Bone Core Sampling - Related Videos
03:10
Soil Core Sample Collection for Water Retention Property Analysis
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2026
Source:Alessia J. Marchesan1, Guillermo Hernandez Ramirez11Renewable Resources, University of Alberta. This video demonstrates the technique for collecting a soil sample for hydraulic property analysis. The sampling area is cleared, and the top crust is evacuated. The sampling ring is then placed into the soil to collect the sample. The ring with the sample is then cleaned, labeled, packed, and transferred undisturbed for analysis.
07:05
A New Workflow for Sampling and Digitizing Increment Cores
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Cited by 11 •
2024
We present a protocol to use 3D-printed mounts to fix increment cores in the field without the necessity of unpacking and gluing them on wooden mounts. The new GSC holder allows placing the cores in a core microtome to cut their surface and directly transfer them to digital image capturing.
01:22
Bone Marrow Sampling and Transplants
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2024
Bone marrow transplant is a potential cure for several diseases, including cancer and specific genetic disorders. Notably, this procedure is applicable for patients suffering from aplastic anemia, certain types of leukemia, severe combined immunodeficiency disease (SCID), Hodgkin's disease, non-Hodgkin's lymphoma, multiple myeloma, thalassemia, sickle-cell disease, and certain cancers. The transplant begins with high doses of chemotherapy and radiation treatment, which aim to destroy the...
07:10
A Sectioning, Coring, and Image Processing Guide for High-Throughput Cortical Bone Sample Procurement and Analysis for Synchrotron Micro-CT
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Cited by 6 •
2020
We employed a geological (coring) sampling protocol to procure cortical bone specimens of uniform size for SRµCT experiments from the anterior aspect of human femora. This method is minimally destructive, efficient, results in cylindrical specimens that minimize imaging artifacts from irregular sample shapes and improves microarchitectural visualization and analysis.
02:10
The Nucleosome Core Particle
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2020
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins. The paradox Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
FAQs
The cylindrical geometry is scientifically valuable because it retains the spatial relationship between mineralized tissue, marrow, and cellular structures. That organization lets investigators examine how these components occur together rather than treating them as isolated materials. In biological studies, this supports interpretation of tissue architecture, remodeling, and mineralization in relation to overall bone function.
Bone core sampling can reveal changes at several structural levels in one specimen. Microscopy may show tissue architecture and cellular organization, while the mineralized portion provides context for mineralization and remodeling. Examining marrow in the same preserved relationship adds information about composition, helping connect local biological changes with skeletal development or disease-related alterations.
Specimen handling determines which features remain available for examination. After removal, the core is stabilized and may undergo fixation, decalcification, sectioning, and microscopy. These stages convert an intact sample into interpretable sections while maintaining a basis for assessing mineralized tissue, marrow, and cellular structures. The preparation sequence therefore supports the intended biological or diagnostic analysis.
A typical workflow begins with cutting the cylindrical specimen using a trephine or similar coring instrument. The core is then stabilized, processed through preparation steps such as fixation or decalcification, sectioned, and examined microscopically. This progression moves from physical collection to tissue visualization, allowing the same sample to support biological, histological, or diagnostic analysis.
Researchers choose this approach when the question depends on relationships within bone. Its cores can support investigations of skeletal development, implant integration, and responses to therapeutic interventions. They can also provide evidence of disease-related changes, making the technique useful across developmental, experimental, and diagnostic contexts. These applications connect microscopic findings with changes in bone health and function.
Microscopic findings can be interpreted by considering architecture, mineralization, remodeling, and marrow composition together. This integrated view helps identify how disease-related changes or therapeutic responses affect bone at the tissue level. It also links local structural observations to broader biological questions, including whether development or implant integration is associated with altered skeletal organization.