Cell Counter
Cell Counter - Related Videos
09:33
Determining Cell Number During Cell Culture using the Scepter Cell Counter
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Cited by 56 •
2010
The Scepter Cell Counter is a handheld automated device that can be used to count cells, monitor cell diameter and volume, and be used to check the health and quality of cellular populations from one culture to the next.
13:16
Determination of Mammalian Cell Counts, Cell Size and Cell Health Using the Moxi Z Mini Automated Cell Counter
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Cited by 18 •
2012
The Moxi Z miniature automated cell counter is a novel instrument that combines the Coulter Principle with patented thin-film sensor technology and a proprietary software algorithm to perform sizing and counting of a broad size range of particles as well as to determine the overall health of monodisperse mammalian cell cultures. This protocol describes the use of this instrument for counting and assessing the health of cell cultures.
07:31
Agarose-Based Model Ecosystem for Cultivating Methanotrophs in a Methane-Oxygen Counter Gradient
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2024
A protocol is described for preparing a simple model ecosystem that recreates the methane-oxygen counter gradient found in the natural habitat of aerobic methane-oxidizing bacteria, enabling the study of their physiology in a spatially resolved context. Modifications to common biochemical assays for use with the agarose-based model ecosystem are also described.
10:34
Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
0 Views •
Cited by 2 •
2010
This procedure describes a quick and easy workflow to introduce siRNA into difficult to transfect cell lines and follow gene expression by real-time PCR. Use of an automated cell counter, multi-well electroporation plate, and automated electrophoresis station provide quick and reliable results without the need for expensive robotic handling.
06:11
An Efficient Method to Obtain Dedifferentiated Fat Cells
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Cited by 4 •
2016
We have modified the conditions for DFAT cell generation and provide herein information regarding the use of an improved growth medium for the production of these cells.
FAQs
Manual counting relies on a hemocytometer grid viewed under a microscope, whereas automated systems identify cells through optical imaging or electrical signals. The measurement approach therefore differs: one depends on visual enumeration across calibrated positions, while the other uses instrument-based detection. Comparing these approaches helps researchers select a workflow suited to the experiment and desired reproducibility.
A viability measurement adds biological status to the total cell count. When paired with suitable dyes, the method can distinguish living cells from damaged cells, helping researchers determine whether a sample contains an effective population rather than only a numerical quantity. This distinction is especially relevant when evaluating treatment effects or preparing cells for later experiments.
The calibrated grid provides a defined area in which cells can be distributed and counted under a microscope. Because the counting region is standardized, researchers can use the observed number of cells to obtain a consistent measurement across samples. This structure supports reproducible cell seeding and improves comparability among cell-based experiments.
A manual workflow distributes the sample across a hemocytometer’s calibrated grid, places it under a microscope, and counts the cells within the defined grid area. An automated workflow instead uses an instrument that detects cells through optical imaging or electrical signals. The resulting measurement can then guide standardized seeding or assessment of culture growth.
Researchers use these measurements when they need consistent cell numbers for seeding, want to monitor changes in culture growth, or need to assess how a treatment affects cells. Incorporating counting into these stages helps standardize experimental inputs and supports reproducibility when comparing conditions, time points, or treatment outcomes in biology research.
Cell-counting measurements support work across microbiology, immunology, and tissue engineering, as well as broader biology research and cell-based experiments. In each setting, the measurement can help establish a consistent starting population, follow culture changes, or evaluate treatment effects. Pairing counts with viability information further clarifies the condition of the measured sample.