Cell Metabolism
Cell Metabolism - Related Videos
10:19
An Introduction to Cell Metabolism
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2023
In cells, critical molecules are either built by joining together individual units like amino acids or nucleotides, or broken down into smaller components. Respectively, the reactions responsible for this are referred to as anabolic and catabolic. These reactions require or produce energy typically in the form of a “high-energy” molecule called ATP. Together, these processes make up “Cell Metabolism,” and are hallmarks of healthy, living cells.JoVE’s introduction to cell metabolism briefly...
05:41
Metabolic Profile Analysis of Zebrafish Embryos
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Cited by 42 •
2013
Zebrafish represent a powerful vertebrate model that has been under-utilised for metabolic studies. Here we describe a rapid way to measure the in vivo metabolic profile of developing zebrafish that allows the comparison of different mitochondrial function parameters between genetically or pharmacologically manipulated embryos, thereby increasing the applicability of this organism.
00:52
What is Metabolism?
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2019
Overview Metabolism represents all of the chemical activity in a cell, including reactions that build molecules (anabolism) and those that break molecules down (catabolism). Anabolic reactions require energy, whereas catabolic reactions provide it. Thus, metabolism describes how cells transform energy through a variety of chemical reactions, which are often made more efficient with the help of enzymes. Metabolism Is the Sum of All of the Chemical Reactions That Take Place in an Organism
08:28
Metabolic Labeling
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2023
Metabolic labeling is used to probe the biochemical transformations and modifications that occur in a cell. This is accomplished by using chemical analogs that mimic the structure of natural biomolecules. Cells utilize analogs in their endogenous biochemical processes, producing compounds that are labeled. The label allows for the incorporation of detection and affinity tags, which can then be used to elucidate metabolic pathways using other biochemical analytical techniques, such as SDS-PAGE...
05:01
Extracellular Purine Metabolism: An HPLC-based Assay to Measure Adenosine Generation and Metabolism in Leukemic Cells
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2023
This video explains a quantitative reverse phase high-performance liquid chromatography (RP-HPLC) method to measure metabolism of adenosine and associated purines generated by consumption of AMP in extracellular environment of leukemic cancer cells. The protocol describes a sensitive, specific, reliable and reproducible RP-HPLC assay developed and validated for the quantification of extracellular purine nucleotides and nucleosides produced by purified chronic lymphocytic leukemia (CLL) cells...
FAQs
Catabolic pathways connect nutrient breakdown to energy capture by generating ATP and reducing equivalents. Glycolysis and the citric acid cycle are named stages in this network, while oxidative phosphorylation uses the resulting reducing equivalents to support further energy production. This arrangement converts nutrient-derived chemical energy into forms that can support maintenance, biosynthesis, and changing cellular demands.
Catabolic pathways release energy and provide precursor molecules, whereas anabolic pathways use that energy and those precursors to build proteins, lipids, nucleic acids, and other cellular structures. Coordination between the two directions helps a cell allocate resources between obtaining energy, producing biomass, maintaining its components, and responding to changing conditions.
Cellular homeostasis depends on maintaining suitable internal conditions while energy needs and environmental conditions change. Metabolic pathways contribute to this balance by adjusting how nutrients are broken down and how cellular components are produced. Studying these responses helps explain how cells maintain function, adapt to changing conditions, and support processes such as growth and differentiation.
Metabolic analysis can help researchers examine how cells obtain energy, generate building materials, and adjust pathway activity during growth, differentiation, adaptation, or altered conditions. These observations connect chemical reactions with broader biological outcomes. The approach therefore supports investigations in physiology, pharmacology, biotechnology, and the development of potential therapeutic strategies.
Disease can involve changes in how cells use energy and allocate precursor molecules for cellular construction. Altered energy use is specifically associated with cancer, diabetes, and metabolic disorders in the provided context. Examining these metabolic changes can help researchers connect abnormal cellular activity with disease processes and inform therapeutic development.
Cell metabolism provides a biochemical context for understanding growth, differentiation, adaptation, and homeostasis. Because these processes depend on both energy supply and the production of cellular components, metabolic studies connect molecular reactions with physiology and disease. This broad relevance makes the subject useful in biology as well as pharmacology, biotechnology, and therapeutic research.