Cell Strainer
Cell Strainer - Related Videos
03:57
T-Cell Enrichment: A Technique to Isolate T-Cells from Mixed Cell Population by Magnetic Separation
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2023
This video describes T-cells enrichment from the spleen of a mouse by negative selection using a magnetic separator. The technique involves targeting the other cells in the sample for depletion using antibodies or ligands directed against specific cell surface antigens.
08:59
Live Imaging of Apoptotic Cell Clearance during Drosophila Embryogenesis
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Cited by 10 •
2013
Here we describe an effective method for studying dynamics of apoptotic cell clearance in vivo. This method employs live Drosophila embryos as a powerful model for monitoring phagocytosis of apoptotic cells using specific labeling of apoptotic cells and phagocytes.
03:10
Isolating Murine Retinal Ganglion Cells Using Fluorescence-Activated Cell Sorting
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2025
This video demonstrates the procedure for isolating murine primary retinal ganglion cells (RGCs) by using flow cytometry. This method allows for the future study of RGCs, with the goal of better understanding the major decline in visual acuity that results from the loss of functional RGCs in neurodegenerative diseases.
05:38
Isolating and Analyzing Cells of the Pancreas Mesenchyme by Flow Cytometry
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Cited by 13 •
2017
Here, we describe a method for the isolation of cells in the pancreas microenvironment from embryonic, neonatal and adult mouse tissue, focusing on the isolation of mesenchymal cells. This method allows profiling of cell gene expression and protein secretion in order to elucidate the extrinsic signals that regulate pancreas development, function, and tumorigenesis.
07:56
Isolation of Skeletal Muscle Satellite Cells for In Vitro Myogenesis Studies
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2026
This protocol provides a standardized method for isolating and enriching primary myoblasts from adult and neonatal mouse skeletal muscle through tissue dissociation, sequential filtration, preplating, and defined culture conditions, enabling the establishment and maintenance of satellite cell-derived myoblast cultures for downstream experimental applications.
FAQs
Pore size determines which components enter the filtrate and which remain retained. A suitable opening allows individual cells and smaller particles to pass while holding back larger aggregates, undigested tissue, and debris. Selecting the pore size therefore influences sample uniformity and determines whether the collected material is appropriate for counting, culture, flow cytometry, or molecular analysis.
Clumps can make a biological sample less uniform and interfere with consistent downstream handling. Filtering removes larger aggregates and tissue remnants, producing a suspension with more evenly distributed cellular material. This improved consistency supports more reliable measurements, particularly when the sample will undergo cell counting, flow cytometry, primary cell culture, or molecular assays.
The mesh allows individual cells and small particles to pass into the filtrate while retaining larger aggregates, undigested tissue, and debris. This size-based separation does not describe the biological identity of each particle; it organizes the specimen according to physical dimensions. The resulting fractions can then be directed toward different analytical or handling steps.
The sample is passed through the mesh so that individual cells and smaller particles enter the filtrate while larger clumps and debris remain behind. The resulting suspension is more uniform for subsequent flow-cytometric handling and measurement. Pore size selection is important because it affects which material reaches the analysis step and how consistently the sample can be processed.
A tissue specimen is directed through the strainer, and the filtrate is collected for further biological work. During this step, the mesh retains larger tissue fragments, undigested material, and aggregates, while smaller cellular components pass through. The collected fraction can then support cell counting, primary cell culture, flow cytometry, or molecular assays, depending on the study.
They are useful whenever a more uniform cellular sample is needed before downstream analysis or handling. Applications described for this device include cell counting, primary cell culture, and molecular assays, in addition to flow cytometry. By reducing clumps and debris, filtration can make these workflows more consistent and improve the efficiency of subsequent sample processing.