Dynamic Cell Manipulation
Dynamic Cell Manipulation - Related Videos
10:27
Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells
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Cited by 7 •
2012
A method for spatio-temporal control of small GTPase activity by light is described. This method is based on rapamycin-induced FKBP-FRB heterodimerization and photo-caging systems. Optimization of light-irradiation enables the spatio-temporally controlled activation of small GTPases at the subcellular level.
23:21
Silicon Microchips for Manipulating Cell-cell Interaction
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Cited by 7 •
2007
This article describes an experimental approach for dynamic regulation of cell-cell interactions between adherent cells on a micrometer scale. Manipulation of intercellular communication between hepatocytes and stromal cell is demonstrated. The developed platform enables investigation of cell-cell interactions in a variety of biological processes, including development and pathogenesis.
13:27
Osteopathic Manipulative Treatment as a Useful Adjunctive Tool for Pneumonia
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Cited by 50 •
2014
Pneumonia is one of the top ten leading causes of death in the U.S. Osteopathic manipulative techniques (OMT) may be utilized as an adjunctive therapy in the treatment of pneumonia to enhance biomechanical and immune function; and ultimately, to reduce hospital stay, duration of antibiotics, incidence of respiratory failure, and mortality. In this video-article, we will review randomized controlled studies on the use of OMT in pneumonia patients, as well as demonstrate specific hands-on...
14:14
Visualization of Cortex Organization and Dynamics in Microorganisms, using Total Internal Reflection Fluorescence Microscopy
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Cited by 12 •
2012
Total Internal Reflection Fluorescence (TIRF) microscopy is a powerful approach to observe structures close to the cell surface at high contrast and temporal resolution. We demonstrate how TIRF can be employed to study protein dynamics at the cortex of cell wall-enclosed bacterial and fungal cells.
15:39
Studying Organelle Dynamics in B Cells During Immune Synapse Formation
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Cited by 8 •
2019
Herein we describe two approaches to characterize cell polarization events in B lymphocytes during the formation of an IS. The first, involves quantification of organelle recruitment and cytoskeleton rearrangements at the synaptic membrane. The second is a biochemical approach, to characterize changes in composition of the centrosome, which undergoes polarization to the immune synapse.
FAQs
Its central mechanism couples a controlled cue to a measurable cellular response. Researchers vary fluid flow, mechanical force, light, electric fields, or extracellular conditions, then track changes in movement, shape, organization, or behavior over time. This time-resolved design helps associate a specific environmental change with cellular adaptation rather than observing behavior without knowing what triggered it.
Physical cues alter the conditions surrounding cells through forces, flow, light, or electric fields, whereas chemical cues change the extracellular environment. Dynamic Cell Manipulation can apply either type in a controlled sequence, allowing researchers to examine how cells respond to distinct classes of stimulation. Comparing these responses helps separate effects associated with environmental chemistry from those associated with physical conditions.
Precise timing establishes when a cellular response begins relative to the applied cue. Real-time imaging or monitoring can then capture progressive changes rather than only a final state. This is particularly useful for examining migration, adhesion, division, communication, and adaptation, because the sequence and duration of responses may reveal how cells reorganize as conditions change.
A typical workflow begins by placing living cells in an environment where a physical or chemical condition can be changed in a controlled manner. Researchers apply the selected cue at a defined time, image or monitor the cells during the intervention, and examine resulting changes in behavior or organization. The outcome is a time-linked record of cellular responses.
Researchers choose this approach when they need to examine how cells respond to changing surroundings rather than simply characterize their appearance. It can reveal dynamic behaviors associated with development, tissue organization, disease progression, or drug responses. Applying a defined cue while monitoring cells also supports comparisons between cellular states before, during, and after environmental change.
The approach can be used to investigate how cells migrate, adhere, divide, communicate, and adapt to their surroundings. These behaviors are relevant to tissue organization and development, as well as disease progression and responses to drugs. It also contributes to engineered cellular systems by providing a way to study how controlled conditions influence cellular organization and function over time.