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Isolating and Analyzing Cells of the Pancreas Mesenchyme by Flow Cytometry
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Isolating and Analyzing Cells of the Pancreas Mesenchyme by Flow Cytometry

Isolating and Analyzing Cells of the Pancreas Mesenchyme by Flow Cytometry

DOI:
10.3791/55344-v

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05:38 min

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January 28, 2017

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, ,
1Cell and Developmental Biology,Sackler Faculty of Medicine, Tel Aviv University

Chapters

  • 00:05Title
  • 00:39Pancreas Digestion and Fluorescence-activated Cell Sorting (FACS)
  • 03:54Results: Representative Isolated Pancreatic Cell Analyses
  • 05:13Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe a method for the isolation of cells in the pancreas microenvironment from embryonic, neonatal and adult mouse tissue, focusing on the isolation of mesenchymal cells. This method allows profiling of cell gene expression and protein secretion in order to elucidate the extrinsic signals that regulate pancreas development, function, and tumorigenesis.

Tags

Keywords: PancreasMesenchymeFlow CytometryCell IsolationGene ExpressionSurface MarkerPancreas DevelopmentEpithelial DevelopmentCell SortingDigestive BufferHBSSCentrifugationCell StrainerSorting BufferViability DyeFACS

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