Colony Dis-adhesion
Colony Dis-adhesion - Related Videos
05:57
In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells
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Cited by 81 •
2011
This protocol is a simple bacterial adhesion assay consisting in counting the numbers of bacterial colony forming units that are adhered onto cultured cells. The assay is robust, independent of the adhesin studied, and numerous variations are used in most laboratories working on bacterial pathogenesis.
01:14
Adhesion
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2019
Adhesion occurs when one type of molecule is attracted to a different molecule. Water exhibits adhesive properties in the presence of polar surfaces, such as glass or cellulose in plants. For instance, when water is poured into a glass, the positively charged hydrogen molecules of water are more attracted to the negatively charged oxygen molecules in the silica than to the oxygen in neighboring water molecules. Capillary action is a result of water’s adhesive tendencies. When a narrow glass...
07:21
Rapid, Cost-Efficient, Enzyme-Free Passaging of Human Pluripotent Stem Cells on Feeder Cells by Ethylenediaminetetraacetic Acid-Mediated Dis-Adhesion
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2023
To avoid the limitations associated with the enzymatic or mechanical passaging of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) cultured on feeder cells, we have established a fast, effective, cost-efficient, high-yield method for harvesting hESC or hiPSC colonies maintained on a feeder cell layer of human foreskin fibroblasts using EDTA-mediated dis-adhesion.
06:44
Keratinocyte Colony Formation Assay: An In Vitro Assay to Assess the Ability of Keratinocytes to Grow into Colonies
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2023
In this video, we perform a clonogenic assay of keratinocytes harvested from adult mice to determine the ability of individual cells to proliferate and form colonies. This keratinocyte harvesting technique is useful for studying other downstream aspects of cutaneous chemical carcinogenesis and tumor promotion.
13:28
Introducing Shear Stress in the Study of Bacterial Adhesion
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Cited by 9 •
2011
During the infection process, a key step is the adhesion of pathogens with host cells. In most instances this adhesion step occurs in the presence of mechanical stress generated by flowing liquid. We describe a technique that introduces shear stress as an important parameter in the study of bacterial adhesion.
FAQs
Cadherins help maintain cell-cell junctions, whereas integrins connect cells to the underlying extracellular matrix. When either contact system changes, neighboring cells can lose cohesion or cells can weaken their attachment to the substrate. These effects are not isolated: cytoskeletal tension and matrix interactions also influence adhesion strength. Tracking these linked changes helps explain whether a colony remains organized or begins to separate.
Cytoskeletal tension can determine how strongly adhesive contacts are maintained or challenged. Changes in tension may alter the behavior of cadherin-mediated junctions and integrin-mediated cell-matrix adhesions, shifting cells from stable organization toward separation. The extracellular matrix contributes to this balance by providing the substrate for integrin contacts. This mechanism makes dis-adhesion a coordinated change in cell architecture, not merely a loss of one bond.
It provides a useful context for examining how organized cell behavior changes when adhesion is disrupted. In epithelial-to-mesenchymal transition studies, researchers can consider altered cadherin- and integrin-mediated contacts, cytoskeletal tension, and matrix interactions alongside the loss of cohesive organization. This connection does not make dis-adhesion identical to the transition; rather, it makes adhesion changes relevant when investigating epithelial remodeling and disease-associated cell behavior.
The desired downstream application determines the extent of separation. If researchers need organized groups, controlled dis-adhesion can produce cell clusters; if they require individual cells, the process can be taken to a single-cell outcome. This choice matters for passaging, differentiation, migration studies, and downstream analysis because these applications may require different degrees of retained cell-cell association.
During routine culture, controlled colony dis-adhesion helps release cells from an organized colony so they can be prepared for passaging or further study. The resulting material may remain clustered or become single-cell, depending on the experimental goal. This workflow also supports differentiation experiments, migration studies, and downstream analyses that require cells to be examined after their original colony organization has been altered.
Altered adhesion is biologically important because it connects cell organization with tissue behavior and disease research. Studies of colony dis-adhesion can inform investigations of wound repair, invasion, and metastasis, while also clarifying how epithelial cell arrangements are remodeled. Examining cadherins, integrins, cytoskeletal tension, and extracellular-matrix interactions together helps relate a cellular adhesion change to broader biological outcomes.