Dendritic Cells Antigen Presentation
Dendritic Cells Antigen Presentation - Related Videos
06:57
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In this antigen-driven colitis model, OT-II CD4+ T cells expressing a red fluorescent protein were adoptively transferred into RAG-/- mice that express a green fluorescent protein in mononuclear phagocytes (MPs). The hosts were challenged with Escherichia coli (E.coli) expressing the ovalbumin protein (OVA) fused to a cyan fluorescent protein (CFP).
01:29
Assessing Impaired MHC-I Antigen Presentation in Immune Cells Infected with Bacteria
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Start with an immune cell culture infected with pathogenic bacteria that form intracellular inclusion bodies.The bacterial infection impairs the antigen presentation pathway in the immune cells.Take an uninfected culture as a control.Next, add a model protein antigen and incubate.Immune cells internalize, process, and present the model antigen-derived peptides on MHC-I molecules.After incubation, wash the cells to remove any unprocessed antigen.Fix the cells, then add a quenching agent to...
18:08
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12:48
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The method described here is a new vesicle isolation protocol, which allows for the purification of the cellular compartments where exogenous antigens are processed by endoplasmic reticulum-associated degradation in cross-presentation.
08:40
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Cited by 20 •
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The most commonly used method for generating large numbers of autologous dendritic cells (DCs) for use in tumor immunotherapy is described. The method uses IL-4 and GM-CSF to differentiate DCs from monocytes. The immature DCs are stimulated to mature and then loaded with antigens before they are injected back into the patient.
FAQs
MHC class II presentation connects captured and processed material with CD4+ T-cell responses, whereas cross-presentation places selected extracellular antigens on MHC class I for CD8+ T-cell responses. This distinction allows dendritic cells to connect external sources of antigen with different lymphocyte populations, broadening the adaptive immune responses that can be initiated.
Maturation changes the functional context in which antigen display occurs. In addition to processing antigen, mature dendritic cells increase costimulatory signaling and promote migration to lymph nodes, where interactions with T lymphocytes can occur. These changes help determine whether captured material leads to an effectively initiated and shaped adaptive immune response.
Dendritic cells can acquire antigen from microbes, damaged cells, or other extracellular material. The source matters because the cell must capture and process proteins before displaying their peptide products, and selected extracellular antigens may enter the cross-presentation route. This enables immune recognition of material that does not originate inside the dendritic cell.
A conceptual analysis follows the material from capture through protein processing, peptide loading, and display on MHC molecules. It then examines costimulatory signaling and migration to lymph nodes as indicators of maturation, followed by the T-cell population involved. This sequence distinguishes antigen handling from the cellular changes that support adaptive immune activation.
Vaccine research can use this process to examine how antigen capture and processing promote T-cell responses. Studies may consider whether material is presented through MHC class II to CD4+ cells or cross-presented through MHC class I to CD8+ cells, while also evaluating maturation, costimulation, and lymph-node migration as important response-shaping features.
The same antigen-presentation framework helps researchers study several outcomes of immune recognition. In infection and cancer, it provides context for responses against microbial or abnormal material; in autoimmunity and tolerance research, it helps examine how antigen handling and T-cell activation are regulated. These applications connect cellular antigen processing with broader immune-response control.