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JoVE Journal
Immunology and Infection

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Preparation of Tumor Antigen-loaded Mature Dendritic Cells for Immunotherapy
 

Preparation of Tumor Antigen-loaded Mature Dendritic Cells for Immunotherapy

Article DOI: 10.3791/50085
August 1st, 2013

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Summary August 1st, 2013

The most commonly used method for generating large numbers of autologous dendritic cells (DCs) for use in tumor immunotherapy is described. The method uses IL-4 and GM-CSF to differentiate DCs from monocytes. The immature DCs are stimulated to mature and then loaded with antigens before they are injected back into the patient.

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