Dna Probe Hybridization
Dna Probe Hybridization - Related Videos
14:57
DNA Stable-Isotope Probing (DNA-SIP)
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Cited by 70 •
2010
DNA stable-isotope probing is a cultivation-independent method to identify and characterize active communities of microorganisms that are capable of utilizing specific substrates. Assimilation of substrate enriched in heavy isotope leads to incorporation of labelled atoms into microbial biomass. Density gradient ultracentrifugation retrieves labelled DNA for downstream molecular analyses.
07:44
Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
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Cited by 5 •
2016
Photostable cyanine dyes are attached to oligonucleotides to monitor hybridization by energy transfer.
12:16
Robust 3D DNA FISH Using Directly Labeled Probes
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Cited by 49 •
2013
We describe a robust and versatile protocol for analyzing nuclear architecture by 3D DNA FISH using directly labeled fluorescent probes.
06:00
HCR-DNA FISH: A Fluorescence In Situ Hybridization Technique to Detect Viral DNA in Infected Cells
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2023
In this video, the cells infected with MCPyV virions were subjected to in situ hybridization chain reaction to detect MCPyV specific genomes. Further, the DNA-HCR technique was combined with FISH to visualize the MCPyV DNA in infected human skin cells.
03:26
Probe Hybridization and Signal Amplification in RNA In Situ Hybridization: A Technique for Detecting Specific RNA Sequences in Tissue Sections
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2023
This video describes a sequential hybridization strategy that involves hybridizing mRNA probes to specific target mRNA. Following probe hybridization, signal amplification is performed to enhance resolution via reduction of signal-to-noise ratio during RNA-CISH of histological samples.
FAQs
Hybridization stringency controls how selectively a probe and target remain paired. Higher stringency favors more specific matching under the selected temperature and salt conditions, while lower stringency permits less restrictive pairing. Probe length also contributes because it affects the sequence available for complementary recognition. Researchers adjust these variables to balance detection of the intended sequence against nonspecific binding.
Probe length influences how much complementary sequence is available for recognition, making it an important variable for tuning specificity. Its effect must be interpreted together with temperature and salt concentration because these conditions jointly establish hybridization stringency. Selecting probe length alongside those settings helps investigators distinguish the intended genetic sequence during molecular analysis.
The label makes a probe-target binding event detectable, allowing investigators to determine whether the complementary sequence is present. Because recognition depends on base pairing, the label serves as the readout rather than the source of sequence specificity. This distinction lets the same underlying approach support genetic analysis, cellular or chromosomal localization, and diagnostic testing.
An assay begins by selecting a DNA fragment whose sequence complements the target and attaching a detectable label. The probe is exposed to the relevant nucleic acid under controlled temperature and salt conditions, allowing complementary pairing to occur. Researchers then interpret the label-associated detection to identify the target sequence. This general workflow can be adapted to several assay formats.
These formats apply the same sequence-recognition principle to different biological questions. Southern blotting supports detection of particular DNA sequences, whereas fluorescence in situ hybridization can locate DNA within cells or chromosomes. Microarrays and diagnostic assays provide additional contexts for genetic analysis and testing. The appropriate format depends on whether the goal is detection, localization, comparison, or diagnosis.
DNA probe hybridization is useful when a research question concerns the presence or position of a defined sequence. It can help identify genes, compare genetic sequences, locate DNA in cells or chromosomes, and detect pathogens or disease-associated mutations. These outcomes connect a molecular assay with broader objectives in genetic research, clinical testing, and investigation of disease-associated variation.