Embryonic Muscle Isolation
Embryonic Muscle Isolation - Related Videos
14:36
Adult and Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells
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Cited by 6 •
2010
The micro-dissected explants technique is a robust and reliable method for isolating proliferative skeletal muscle cells from juvenile, adult or embryonic muscles as a source of skeletal muscle stem cells. Uniquely, these cells have been clonally derived to produce skeletal muscle stem cell lines used for in vivo transplantation.
08:05
Isolation of Embryonic Ventricular Endothelial Cells
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Cited by 4 •
2013
Primary cell culture is a useful technique for analyzing specific populations of cells, particularly from transgenic mouse embryos at specific developmental stages. Herein, embryonic ventricles are dissected and dissociated, and antibody-conjugated beads recognize and separate out the endothelial cells for further analysis.
14:02
Ex Vivo Assessment of Contractility, Fatigability and Alternans in Isolated Skeletal Muscles
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Cited by 53 •
2012
We describe a method to directly measure muscle force, muscle power, contractile kinetics and fatigability of isolated skeletal muscles in an in vitro system using field stimulation. Valuable information on Ca2+ handling properties and contractile machinery of the muscle can be obtained using different stimulating protocols.
07:13
Laser-inflicted Injury of Zebrafish Embryonic Skeletal Muscle
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Cited by 14 •
2013
The method presented here comprises the precise injury of live zebrafish embryos with high-energy laser pulses and the subsequent analysis of these injuries and their recovery with time. We also show how genetically labeled single or groups of skeletal muscle cells can be tracked during and after laser light induced damage.
09:45
Mitochondrial Isolation from Skeletal Muscle
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Cited by 47 •
2011
This protocol describes a procedure to study the respiration of mitochondria isolated from skeletal muscles. This method was adapted from Scorrano et al. (2007). The mitochondrial isolation procedure requires about 2 hours. The mitochondrial respiration can be completed in about 1 hour.
FAQs
Mechanical dissociation physically separates developing tissue, while enzymatic digestion helps release individual myoblasts from surrounding cellular connections. Using both steps can improve cell recovery compared with relying on one approach alone, but the process must remain sufficiently controlled to preserve viability. The resulting cell suspension provides a suitable starting material for subsequent culture and developmental analysis.
Controlled culture conditions help isolated myoblasts remain viable while supporting two important developmental outcomes: proliferation and differentiation into muscle fibers. This makes it possible to observe changes over time rather than examining only the initial cell population. Researchers can therefore connect environmental conditions with the progression of muscle formation and assess cellular responses during myogenesis.
These cells provide an experimental system for examining myogenesis, the developmental formation of muscle, together with cell signaling and gene regulation. Because the cells can be studied during proliferation and differentiation, researchers can investigate how molecular controls influence tissue formation. The system links cellular behavior with broader developmental events that may be difficult to resolve in intact embryonic tissue.
A typical workflow begins with precise dissection of the developing muscle region from an embryo. The recovered tissue then undergoes mechanical dissociation and enzymatic digestion to release myoblasts, followed by transfer into controlled culture conditions. Researchers subsequently monitor the isolated cells as they proliferate and differentiate, using the cultured population for developmental or cellular analyses.
Viability determines whether isolated cells can continue the behaviors that make the preparation informative. Cells that remain healthy after dissection and dissociation can proliferate, differentiate, and support measurements of signaling or gene regulation. Consequently, the isolation process is not judged only by releasing cells from tissue; it must also maintain a population capable of responding in culture.
The technique is useful when researchers need a tractable cellular model for studying muscle development, regeneration, disease mechanisms, or potential therapeutic strategies. Cultured embryonic muscle cells allow investigators to examine developmental behavior under controlled conditions and relate cellular findings to tissue formation. This connects basic biology with questions about how muscle may be repaired or altered in disease.