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Mitochondrial Isolation from Skeletal Muscle
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Mitochondrial Isolation from Skeletal Muscle

Mitochondrial Isolation from Skeletal Muscle

DOI:
10.3791/2452-v

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09:45 min

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March 30, 2011

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, ,
1Department of Physiology,University of Kentucky

Chapters

  • 00:05Title
  • 01:15Muscle Isolation/Homogenization and Mitochondrial Isolation
  • 04:13Electrode Calibration
  • 06:17Mitochondrial Respiration
  • 08:15Representative Mitochondrial Respiration Results
  • 09:15Conlcusion

Summary

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This protocol describes a procedure to study the respiration of mitochondria isolated from skeletal muscles. This method was adapted from Scorrano et al. (2007). The mitochondrial isolation procedure requires about 2 hours. The mitochondrial respiration can be completed in about 1 hour.

Tags

Mitochondrial IsolationSkeletal MuscleOrganellesMetabolic ReactionsATP SynthesisSignaling CascadesLiverBrainHeartSkeletal Muscle FunctionIsolation MethodBuffersReagentsMitochondrial Protein ConcentrationFunctional StudiesMitochondrial RespirationPolarographyClark-type ElectrodeOxygen Electrode CalibrationExperimental BufferGlutamateMalateAdenosine Diphosphate (ADP)Oligomycin

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