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Chapters
- 00:00Title
- 01:51Introduction
- 02:38Establishing Primary Skeletal Muscle Microexplant Cultures
- 05:33Establishing Primary Microexplant Cultures from Embryos
- 09:43 in vitro Analysis of Skeletal Muscle Stem Cells and Primary Cultures
- 11:15Representative Results of Skeletal Muscle Microexplant Culture
- 14:01Conclusion
The micro-dissected explants technique is a robust and reliable method for isolating proliferative skeletal muscle cells from juvenile, adult or embryonic muscles as a source of skeletal muscle stem cells. Uniquely, these cells have been clonally derived to produce skeletal muscle stem cell lines used for in vivo transplantation.
Tags
Skeletal Muscle Microexplant CultureIsolation Of Skeletal Muscle Stem CellsEmbryonic Skeletal Muscle CellsAdult Skeletal Muscle CellsMicro-dissected Explants TechniqueProliferative Skeletal Muscle CellsGrowth CharacteristicsCell SurvivalProliferationSenescenceDifferentiationExplant Outgrowth RatesPrimary CulturesEmbryonic Skeletal Muscle PrecursorsClonal SMSc LinesIn Vivo TransplantationSatellite CellsSkeletal Muscle Fibre Regeneration
