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September 21, 2010
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Summary
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The micro-dissected explants technique is a robust and reliable method for isolating proliferative skeletal muscle cells from juvenile, adult or embryonic muscles as a source of skeletal muscle stem cells. Uniquely, these cells have been clonally derived to produce skeletal muscle stem cell lines used for in vivo transplantation.
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Cite this Article
Merrick, D., Chen, H., Larner, D., Smith, J. Adult and Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells. J. Vis. Exp. (43), e2051, doi:10.3791/2051 (2010).
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