Epidermal Cell Migration
Epidermal Cell Migration - Related Videos
08:29
Mouse Epidermal Neural Crest Stem Cell (EPI-NCSC) Cultures
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Cited by 15 •
2008
Here we show our method to isolate mouse epidermal neural crest stem cells (EPI-NCSC). Technique involves micro-dissecting whisker follicles, isolating the bulge and placeing it into tissue culture. EPI-NCSC start to emigrate from bulge explants onto the substratum within 3 - 4 days.
21:16
An In Vitro Skin Irritation Test (SIT) using the EpiDerm Reconstructed Human Epidermal (RHE) Model
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Cited by 57 •
2009
In this video, we demonstrate the EpiDerm Skin Irritation test (EpiDerm SIT) developed and validated for in vitro skin irritation testing of chemicals, including cosmetic and pharmaceutical ingredients.
08:52
An Introduction to Cell Motility and Migration
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2023
Cell motility and migration play important roles in both normal biology and in disease. On one hand, migration allows cells to generate complex tissues and organs during development, but on the other hand, the same mechanisms are used by tumor cells to move and spread in a process known as cancer metastasis. One of the primary cellular machineries that make cell movement possible is an intracellular network of myosin and actin molecules, together known as “actomyosin”, which creates a...
06:51
Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin
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Cited by 23 •
2016
An ideal model for studying adult stem cell biology is the mouse hair follicle. Here we present a protocol for isolating different populations of hair follicles stem cells and epidermal keratinocytes, employing enzymatic digestion of mouse dorsal skin followed by FACS analysis.
09:36
Study of Cell Migration in Microfabricated Channels
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Cited by 43 •
2014
A quantitative method to study spontaneous migration of cells in a one-dimensional confined microenvironment is described. This method takes advantage of microfabricated channels and can be used to study migration of large number of cells under different conditions in single experiments.
FAQs
Movement depends on coordinated remodeling of the cytoskeleton. Epidermal cells extend actin-rich protrusions, establish traction through attachments to extracellular matrix proteins, and use those forces to advance across the damaged area. This coordination links internal actin organization with external substrate engagement, helping explain how cellular activity contributes to re-epithelialization during wound repair.
Integrins function as attachment components that connect epidermal cells with extracellular matrix proteins. These contacts provide the physical engagement needed for traction, allowing cytoskeletal remodeling to support movement. Their role is therefore distinct from cell-cell adhesion: integrins link cells to the matrix, while other adhesion changes coordinate relationships among neighboring cells.
Cell-cell adhesion must be remodeled, rather than treated as fixed, during repair. This change allows keratinocytes to coordinate movement across the damaged surface while their cytoskeleton reorganizes and integrin-mediated contacts provide traction. The balance matters because successful closure requires both controlled interactions among neighboring cells and productive engagement with the extracellular matrix.
Researchers can assess the process by asking whether coordinated movement leads to re-epithelialization and restoration of the skin barrier. These outcomes connect cellular behavior with tissue-level repair: migration is not only movement across a surface, but also a contributor to closing the damaged area and recovering protective function.
In biology research, the process provides a framework for examining wound-healing mechanisms, modeling skin disease, and assessing regenerative therapies. Studying how cells remodel adhesion, protrusions, cytoskeletal organization, and matrix attachment can help relate cellular migration to tissue repair. Its value spans basic studies of repair and evaluation of approaches intended to restore damaged skin.
Defects in epidermal cell migration can disrupt the progression from cellular movement to tissue closure and barrier recovery. The overview links such defects with chronic wounds and abnormal tissue repair, making migration relevant to disease investigation. Understanding where coordination fails can help frame research into why repair is incomplete or improperly organized.