Epithelial Stem Cells
Epithelial Stem Cells - Related Videos
10:48
Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells
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Cited by 53 •
2017
This protocol describes how to produce retinal pigment epithelial cells (RPE) from pluripotent stem cells. The method uses a combination of growth factors and small molecules to direct the differentiation of stem cells into immature RPE in fourteen days and mature, functional RPE after three months.
08:26
Isolation and Culture of Adult Epithelial Stem Cells from Human Skin
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Cited by 18 •
2011
A rapid, robust way of isolating viable adult epithelial stem cells from human skin is described. The method utilizes enzymatic digestion of skin collagen matrix , followed by plucking of hair follicles and isolation of single cell suspensions or tissue fragments for cell culture.
12:08
Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells
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Cited by 5 •
2016
This publication demonstrates methods for successful sampling and culture of nasal epithelial mucosa from children, and reprogramming these cells to induced Pluripotent Stem Cells (iPSCs).
08:14
Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor
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Cited by 24 •
2014
The continuously growing mouse incisor provides a model for studying renewal of dental tissues from dental epithelial stem cells (DESCs). A robust system for consistently and reliably obtaining these cells from the incisor and expanding them in vitro is reported here.
22:06
Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
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Cited by 8 •
2007
This protocol details the derivation of transplantable hematopoietic stem cells from mouse embryonic stem cells (ESC) and their subsequent injection into lethally irradiated recipient mice. Briefly, ESC are differentiated as embryoid bodies, which are then infected with retroviral HoxB4 and co-cultured with OP9 stromal cells and hematopoietic cytokines.
FAQs
Signals from the surrounding niche help determine how epithelial stem cells balance self-renewal with production of differentiated cells. By regulating proliferation, differentiation, and tissue organization, the niche links cell behavior to the needs of the epithelial tissue. This local control is important because renewal must preserve an organized barrier rather than simply increase cell number.
Asymmetric division can maintain a stem-cell population while producing a cell that contributes to tissue renewal, whereas symmetric division can expand stem or progenitor populations. These alternatives give epithelial tissues flexibility during normal maintenance and repair. Their balance helps determine whether renewal preserves existing organization or increases the available pool of cells after tissue demands change.
After injury, controlled stem-cell renewal can replenish epithelial cells while supporting tissue organization and barrier restoration. The outcome depends on coordinated proliferation, differentiation, and signals from the surrounding niche. Studying these relationships helps biology researchers examine how tissues recover without separating cell replacement from the structural arrangement required for a functional epithelial surface.
Organoid research provides a way to study epithelial stem-cell renewal, differentiation, and tissue organization in a model of epithelial development and maintenance. These systems can help investigators examine how cells respond to regulatory signals and how tissue structures are preserved or disrupted. The resulting insight supports research on normal biology, disease mechanisms, and regenerative strategies.
Epithelial stem cells are relevant to disease modeling because their regulated renewal and differentiation can be examined when tissue organization or barrier maintenance is disrupted. This work helps connect cellular behavior with chronic disease and cancer mechanisms. Comparing normal and disease-related patterns can clarify how altered control of epithelial tissues contributes to pathology.
Epithelial stem-cell biology can inform regenerative medicine when damaged epithelial tissues require restored renewal, organization, and barrier function. Understanding niche signals and division patterns may guide therapies designed to support replacement of injured cells while maintaining tissue structure. These principles are relevant to approaches that seek to restore damaged epithelia rather than only relieve symptoms.