Fallopian Tubes
Fallopian Tubes - Related Videos
11:46
Ex Vivo Culture of Primary Human Fallopian Tube Epithelial Cells
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Cited by 29 •
2011
The fallopian tube (FT) is emerging as an alternative site of origin for serous ovarian carcinoma (SOC). This protocol describes a novel method for the isolation and ex vivo culture of fallopian tube epithelial cells. This system recapitulates the in vivo epithelium and allows the study of SOC pathogenesis.
09:11
A Human Fallopian Tube Model for Investigation of C. trachomatis Infections
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Cited by 17 •
2012
We describe an ex vivo infection model for visualisation of direct interactions from bacterial pathogens with human fallopian tube cells. The whole organ tissue model was established to investigate C. trachomatis induced pathology to the female fallopian tube under "life-like" conditions.
08:09
Tubal Cytology of the Fallopian Tube as a Promising Tool for Ovarian Cancer Early Detection
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Cited by 11 •
2017
We explored a tubal cytologic method by sampling the fallopian tube directly post-surgical excision as a tool of ovarian cancer early detection. Here, we present a protocol to collect fallopian tube cells from freshly received surgical specimens.
06:51
Development of Mouse-Derived Organoid Lines from Fallopian Tube Epithelial Cells for High Grade Serous Ovarian Carcinoma Modeling
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2025
This protocol details the development of high-grade serous ovarian carcinoma (HGSOC) mouse models in vitro and in vivo. From the derivation of murine fallopian tube organoids, their genetic modification to recapitulate human HGSOC genetics, and their introduction in the ovarian bursa of syngeneic mice for in vivo development of tumors.
07:03
An Efficient Method for Extracting Human Fallopian Tube Epithelia for Single-cell Analyses
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2025
This protocol describes a one-day method for the isolation of human fallopian tube epithelial cells. Isolated epithelial cells can be plated in 2-dimensional (2D) culture or dissociated into single-cell suspensions and utilized in downstream experiments, including flow cytometry and single-cell RNA sequencing.
FAQs
Fimbriae help guide the released oocyte into the opening of the tube after ovulation. This capture step is essential because the oocyte must enter the tubal environment before it can encounter sperm. Their function links ovarian release with subsequent transport, so impaired capture could disrupt the normal sequence leading to fertilization and early embryonic development.
Ciliated epithelial cells and smooth-muscle contractions provide complementary forces for movement through the tube. Cilia contribute to directed transport, while muscular activity helps propel tubal contents. This coordination must accommodate movement in opposite directions: sperm travel toward the oocyte, whereas the oocyte and early embryonic cells move toward the uterus.
Successful reproduction depends on more than physical passage through the tube. The oocyte, sperm, tubal epithelial cells, and surrounding tissues interact within a coordinated reproductive environment. These relationships support the timing and movement required for fertilization and early development, making tubal biology important for understanding how cellular events are integrated during reproduction.
After ovulation, fimbriae help capture the oocyte, and coordinated ciliary activity and smooth-muscle contractions move it through the tube. Sperm travel in the opposite direction, creating an opportunity for fertilization within the tubal environment. If fertilization occurs, early embryonic cells are subsequently transported toward the uterus.
Blockage or inflammation can interfere with the movement of reproductive cells through the tubes. Such disruption may prevent sperm from reaching the oocyte, hinder oocyte or embryo transport, or disturb the tubal environment needed for normal reproductive progression. Consequently, tubal health is a major consideration when investigating impaired fertility.
The tubes are clinically important because abnormal transport can cause an ectopic pregnancy, in which early development occurs outside the expected uterine location. Studying tubal structure, movement, inflammation, and blockage helps researchers examine how transport becomes disrupted. This connects basic reproductive biology with investigations of fertility problems and pregnancy-related complications.