Fecal Microbiota Transplant
Fecal Microbiota Transplant - Related Videos
03:33
Evaluating the Effects of Fecal Microbiota Transplantation in an Interleukin-10 Deficient Mouse
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2025
This video demonstrates a procedure of fecal microbiota transplantation in an interleukin-10 deficient mouse with colon inflammation. Colonized fecal microbes in the gut release secondary metabolites, eliciting anti-inflammatory cytokine release and reducing colon inflammation.
07:32
Murine Fecal Isolation and Microbiota Transplantation
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Cited by 4 •
2023
The goal here is to outline a protocol to investigate the mechanisms of dysbiosis in cardiovascular disease. This paper discusses how to aseptically collect and transplant murine fecal samples, isolate intestines, and use the "Swiss-roll" method, followed by immunostaining techniques to interrogate changes in the gastrointestinal tract.
07:06
Fecal Microbiota Transplantation via Colonoscopy for Recurrent C. difficile Infection
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Cited by 26 •
2014
Fecal Microbiota Transplantation via colonoscopy is a safe and effective treatment for recurrent and refractory C.difficile infection. A systematic approach to patient and donor screening, preparation of stool, and delivery of the stool during the colonoscopy will maximize therapeutic success.
05:41
Therapeutic Evaluation of Fecal Microbiota Transplantation in an Interleukin 10-Deficient Mouse Model
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Cited by 6 •
2022
The interaction of genetic susceptibility, mucosal immunity, and intestinal microecological environment is involved in the pathogenesis of inflammatory bowel disease (IBD). In this study, we applied fecal microbiota transplantation to IL-10 deficient mice and investigated its impact on colonic inflammation and heart function.
07:54
A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice
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Cited by 9 •
2017
Bacteriocins are believed to play a key role in defining microbial diversity in different ecological niches. Here, we describe an efficient procedure to assess how bacteriocins affect gut microbiota composition in an animal model.
FAQs
Successful colonization depends on more than introducing donor organisms. Recipient biology, the existing intestinal community, and the treatment protocol all influence whether transferred microbes establish themselves. When colonization occurs, donor organisms may compete with pathogens and modify microbial activity in the gut. These variables help explain why comparable interventions can produce different biological outcomes among recipients.
Donor screening and microbial composition are central variables because the transferred community is not identical from one donor to another. Screening helps determine which donors are appropriate, while composition influences the organisms and functions introduced into the recipient. Studying these factors allows researchers to connect variation in the starting microbial community with differences in colonization and treatment outcomes.
Transferred organisms can influence the gut through several linked mechanisms. They may compete with pathogens, produce metabolites, and interact with host immune pathways. These activities connect microbial community changes with biological effects in the recipient. For biology research, Fecal Microbiota Transplant provides a way to examine how microbial functions and host responses interact rather than treating the microbiome as an isolated population.
FMT can be delivered through capsules, an enema, or an endoscopic procedure after donor material has been processed. The selected route is one component of the treatment protocol, alongside donor characteristics and recipient biology. Comparing these variables helps investigators evaluate how delivery conditions influence microbial establishment and the resulting response.
Recurrent Clostridioides difficile infection is the established treatment context identified for FMT. Researchers are also studying its use in inflammatory bowel disease and other conditions associated with microbial dysbiosis, meaning an altered microbial community. These applications test whether changing the intestinal microbiome can produce useful biological effects beyond the condition for which the intervention is established.
FMT provides a model for investigating host-microbe interactions because it changes the microbial community while allowing researchers to examine recipient responses. Its outcomes can be considered alongside donor microbial composition, colonization, pathogen competition, metabolite production, immune-pathway interactions, and protocol design. This integrated perspective supports research into microbiome-based therapies and the biology of dysbiosis.