Femto Jet Injector
Femto Jet Injector - Related Videos
13:29
Jet Impinging on an Inclined Plate
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2023
Source: Ricardo Mejia-Alvarez and Hussam Hikmat Jabbar, Department of Mechanical Engineering, Michigan State University, East Lansing, MI The goal of this experiment is to demonstrate how a fluid flow exerts forces on structures by conversion of dynamic pressure into static pressure. To this end, we will make a plane jet impinge on a flat plate and will measure the resulting pressure distribution along the plate. The resultant force will be estimated by integrating the product between the...
08:35
Lipid Bilayer Vesicle Generation Using Microfluidic Jetting
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Cited by 16 •
2014
Microfluidic jetting against a droplet interface lipid bilayer provides a reliable way to generate vesicles with control over membrane asymmetry, incorporation of transmembrane proteins, and encapsulation of material. This technique can be applied to study a variety of biological systems where compartmentalized biomolecules are desired.
01:14
Free Jet
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2026
Free jets describe the flow of liquid exiting a reservoir through an opening into the atmosphere without resistance. The velocity (v) of the liquid jet is derived using Bernoulli's principle and expressed as: Where h is the height of the liquid above the opening. This relationship assumes atmospheric pressure at both the reservoir surface and the jet exit. The fluid column's height governs the fluid's velocity at the nozzle in a reservoir, where the fluid's potential energy converts into...
08:21
Permeabilization of Adhered Cells Using an Inert Gas Jet
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Cited by 3 •
2013
This protocol describes a method for the temporary permeabilization of adherent cells using an inert gas jet. This technique facilitates the transfer of genetic material and biomolecules into adherent mammalian cells by the utilization of mechanical forces to disrupt the plasma membrane.
08:34
Visualization of High Speed Liquid Jet Impaction on a Moving Surface
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Cited by 4 •
2015
Two experimental devices for examining liquid jet impingement on a high-speed moving surface are described: an air cannon device and a spinning disk device. The apparatuses are used to determine optimal approaches to the application of liquid friction modifier (LFM) onto rail tracks for top-of-rail friction control.
FAQs
Regulated pneumatic pressure drives material through the fine glass micropipette, while injection pressure and duration determine how much enters the target. Higher or longer delivery settings can alter flow, so researchers tune both variables to obtain controlled volumes. This parameter-based control is important when individual cells or embryos must receive material without compromising experimental consistency.
Compensation pressure helps manage fluid movement at the micropipette tip between injections. Adjusting it can limit unwanted leakage, while appropriate injection pressure and duration help reduce excessive delivery and cellular damage. Together, these settings balance reliable transfer with the tolerance of the biological target, making compensation pressure an important control rather than a secondary adjustment.
The fine glass micropipette provides a narrow delivery path suited to very small, controlled volumes. Its use supports targeting individual cells or embryos rather than applying material broadly across a biological sample. In this way, the micropipette connects physical delivery precision with experiments that require manipulation of specific biological systems.
During an injection, the operator regulates pneumatic pressure and sets the injection duration to control delivery. Compensation pressure is also adjusted to limit leakage, while the combined settings help reduce cellular damage. This coordinated control provides a reproducible way to deliver DNA, RNA, proteins, or other reagents to the selected biological target.
The instrument can deliver DNA, RNA, proteins, and other reagents directly into individual cells or embryos. This range supports experiments that require either genetic material or functional molecular components to reach a defined biological target. As a result, the same delivery approach can contribute to cell biology, developmental studies, transgenesis, and gene-editing research.
Direct delivery into embryos enables researchers to examine how introduced DNA, RNA, proteins, or other reagents affect development and supports transgenesis experiments. In cell biology and disease-related studies, targeted manipulation helps investigate cellular function and disease mechanisms. Precise, reproducible delivery strengthens comparisons between experimental conditions by improving control over what each target receives.