Fibroblast Transduction
Fibroblast Transduction - Related Videos
11:48
Generation of Induced Pluripotent Stem Cells by Reprogramming Mouse Embryonic Fibroblasts with a Four Transcription Factor, Doxycycline Inducible Lentiviral Transduction System
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Cited by 22 •
2009
The Stemgent Dox Inducible Mouse TF Lentivirus Set can reprogram mouse embryonic fibroblasts (MEFs) to induced pluripotent stem (iPS) cells. Here we demonstrate the protocol for DOX-inducible expression of mouse reprogramming transcription factors Oct4, Sox2, Klf4 and c-Myc to generate iPS colonies that express common mES pluripotency markers.
15:43
Experimental Generation of Carcinoma-Associated Fibroblasts (CAFs) from Human Mammary Fibroblasts
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Cited by 25 •
2011
Carcinoma-associated fibroblasts (CAFs) rich in myofibroblasts present within the tumour stroma, play a major role in driving tumour progression. We developed a coimplantation tumour xengraft model for experimentally generating CAFs from human mammary fibroblasts. The protocol describes how to establish CAF myofibroblasts that acquire an ability to promote tumourigenesis.
03:32
Generating Neurons by Reprogramming Human Dermal Fibroblast Cells
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2025
The video demonstrates the generation of neurons from adult human dermal fibroblasts (aHDFs). The aHDFs are reprogrammed using a lentiviral vector carrying genes for neuronal transcription factors and short hairpin RNAs (shRNAs). The shRNAs inhibit the expression of a neuronal transcriptional repressor, and the neuronal transcription factors activate genes to differentiate aHDFs into neurons. These reprogrammed aHDFs are cultured in an early and a late neuronal conversion medium to generate...
01:16
Transduction
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2025
Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome are...
03:13
In Utero Viral Transduction in a Mouse
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2025
Source: Lutz, D., et al. Assessment of Ultrastructural Neuroplasticity Parameters After In Utero Transduction of the Developing Mouse Brain and Spinal Cord. J. Vis. Exp. (2019).This video demonstrates viral transduction via in-utero injection in mouse embryos. A pregnant mouse is prepared for the surgery, and a midline incision is made to expose the uterine horns. A mixture of a virus containing the desired gene and a tracking dye is injected into the embryos within the uterine sacs at the...
FAQs
Viral vectors initiate transduction by binding receptors on the fibroblast surface, a recognition step that enables entry into the cell. After entry, the vector delivers DNA or RNA rather than merely attaching to the membrane. The delivered genetic material can then drive expression of a chosen gene, allowing investigators to change fibroblast behavior or mark the cells for study.
Whether expression is transient or stable depends largely on the vector and on whether the delivered payload integrates into the fibroblast genome. Transient expression provides a temporary genetic effect, whereas integration can support longer-lasting expression. This distinction matters when designing experiments that require either a short observation window or a persistent cell model.
Fibroblast transduction is especially informative because these cells produce extracellular matrix and help support tissue structure. Introducing genetic material can therefore let researchers examine how altered gene activity affects matrix production or wound-healing processes. In biology, the method connects cell-level genetic manipulation with changes in tissue-support functions, rather than treating fibroblasts only as passive recipient cells.
A conceptual workflow starts with fibroblasts and a selected viral vector carrying DNA or RNA. The vector binds cell-surface receptors, enters the cells, and delivers its payload. Researchers then interpret the resulting expression according to the experimental goal, asking whether the effect is transient or stable and whether the cells are labeled, altered, or used to generate a specialized model.
Researchers select this approach when they need to label fibroblasts, modify gene activity, or create a specialized cell model. Labeling supports cell identification, while gene alteration enables direct study of cellular functions. Specialized models can address particular biological questions, making transduction useful for experiments focused on fibroblast behavior, extracellular-matrix production, wound healing, and related tissue processes.
Beyond gene-expression studies, fibroblast transduction contributes to cellular reprogramming and tissue-engineering research. It also supports investigations of disease mechanisms and the development of regenerative strategies. These applications extend the method from manipulating fibroblast genetics to examining how changes in these connective-tissue cells may inform tissue repair, extracellular-matrix organization, and broader approaches to restoring tissue structure.