Gap Repair
Gap Repair - Related Videos
04:51
Q Suture Technique: A Suturing Technique to Reduce Gap Formation and Increase Tensile Strength in Tendon Repair
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2025
In this video, we demonstrate suturing techniques — core suture and Q suture — to repair the foot flexor tendon in an ex vivo porcine model. Q sutures enhance the strength of the core suture in tendon repair and decrease gapping at the tendon repair site.
01:54
Base Excision Repair
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2020
One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template. The first step of...
10:32
Using Q Suture to Enhance Resistance to Gap Formation and Tensile Strength of Repaired Flexor Tendons
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Cited by 1 •
2020
Here, we present a “Q” suture technique that can be performed in tendon repair and its effects on the gap formation and tensile strength of the repaired tendons. Q suture is shown to be efficient in enhancing the tensile resistance and tendon repair strength.
10:32
Detection of Post-Replicative Gaps Accumulation and Repair in Human Cells Using the DNA Fiber Assay
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Cited by 3 •
2022
Here we describe two modifications of the DNA fiber assay to investigate single-stranded DNA gaps in replicating DNA after lesion induction. The S1 fiber assay enables the detection of post-replicative gaps using the ssDNA-specific S1 endonuclease, while the gap-filling assay allows visualization and quantification of gap repair.
01:37
Gap Junctions
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2026
Multicellular organisms employ a variety of ways for cells to communicate with each other. Gap junctions are specialized proteins that form pores between neighboring cells in animals, connecting the cytoplasm between the two, and allowing for the exchange of molecules and ions. They are found in a wide range of invertebrate and vertebrate species, mediate numerous functions including cell differentiation and development, and are associated with numerous human diseases, including cardiac and...
FAQs
The repair process uses sequence information from an undamaged complementary strand or a homologous DNA template. DNA polymerase follows that available template to add the missing nucleotides, allowing the repaired region to match the intended sequence. This template-guided mechanism helps preserve genome integrity rather than filling the gap without sequence information.
DNA polymerase restores the missing nucleotides by extending from the existing DNA end and using a complementary template for guidance. Filling the gap does not necessarily make the DNA backbone continuous, so DNA ligase seals the remaining nick. Together, these activities complete both sequence restoration and structural continuity.
Small missing regions can be addressed through template-guided nucleotide addition, whereas larger gaps may require homologous recombination. This process uses matching DNA information from a homologous template to support restoration across a broader region. Studying that distinction connects gap repair with replication, DNA damage responses, and mechanisms that maintain genome stability.
A laboratory study can begin with a DNA molecule containing a defined missing region or an incompletely replicated segment. Researchers then provide or examine an undamaged complementary or homologous template, allow repair-associated DNA synthesis and sealing to occur, and analyze whether the intended recombinant DNA structure was restored. The design can test repair efficiency and accuracy.
Gap repair supports recombinant DNA assembly by allowing missing or discontinuous sequence regions to be restored using matching DNA information. Researchers can use this principle to join or complete designed DNA molecules, then evaluate whether the intended construct formed. The approach links a natural genome-maintenance mechanism with laboratory construction of recombinant DNA.
Experiments can measure the accuracy and efficiency of repair, providing complementary information about whether the correct sequence was restored and how effectively the missing region was completed. These measurements help researchers compare repair pathways, investigate responses to DNA damage, and examine how replication-associated problems affect genome stability.