Stem Cell Adhesion
Stem Cell Adhesion - Related Videos
22:06
Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
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Cited by 8 •
2007
This protocol details the derivation of transplantable hematopoietic stem cells from mouse embryonic stem cells (ESC) and their subsequent injection into lethally irradiated recipient mice. Briefly, ESC are differentiated as embryoid bodies, which are then infected with retroviral HoxB4 and co-cultured with OP9 stromal cells and hematopoietic cytokines.
06:47
Microfluidic Assay for the Assessment of Leukocyte Adhesion to Human Induced Pluripotent Stem Cell-derived Endothelial Cells (hiPSC-ECs)
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Cited by 6 •
2018
This step-by-step protocol provides a detailed description of the experimental setup and data analysis for the assessment of inflammatory responses in hiPSC-ECs and the analysis of leukocyte adhesion under physiological flow.
07:29
Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies
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Cited by 27 •
2015
Pancreatic cancer stem cells (CSCs) can be expanded in vitro using the anchorage-independent sphere culture technique, which represents a powerful tool to study CSC biology and can serve as the first step to develop novel CSC-targeting therapies. Here the methodology for expanding, analyzing and targeting of pancreatic CSCs is provided.
10:24
Quantitation of Endothelial Cell Adhesiveness In Vitro
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Cited by 10 •
2015
We report an in vitro method that allows the quantitation of the actual number of adhesive cells within an endothelial cell monolayer.
20:38
Isolation and Transplantation of Hematopoietic Stem Cells (HSCs)
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Cited by 31 •
2007
FAQs
Integrins connect stem cells with extracellular-matrix components, whereas cadherins support adhesion between neighboring cells. These receptor interactions assemble signaling complexes at the cell surface and link external contacts to the cytoskeleton. Consequently, adhesion is not merely physical: it provides positional information that can influence how stem cells organize and behave within tissues.
Connections to the cytoskeleton allow adhesive contacts to affect cell polarity, migration, survival, self-renewal, and differentiation. Polarity helps establish organized orientation, while migration changes where cells reside; survival and self-renewal influence population maintenance, and differentiation changes cell fate. Studying these linked outcomes explains how local adhesive signals can produce broader biological effects.
Matrix attachment and cell-cell adhesion provide distinct types of environmental input. Integrin-mediated contacts with matrix components can be considered alongside cadherin-mediated interactions with adjacent cells, rather than as interchangeable events. Examining both is important because stem cells experience supportive niche interactions that help organize tissue structures and regulate behavior.
Within the stem cell niche, adhesive contacts help preserve relationships between stem cells, neighboring cells, and the surrounding matrix. Those relationships create signaling contexts that can influence survival, self-renewal, migration, polarity, and differentiation. Niche-focused analysis therefore connects local tissue organization with the maintenance or changing state of stem cell populations.
In developmental research, adhesion studies can clarify how stem cells organize into tissue structures and respond to their local surroundings. In disease modeling, the same framework helps investigators examine altered relationships among cells, matrix components, signaling complexes, and the cytoskeleton. These observations connect cellular behavior with changes in tissue organization and cell fate.
Regenerative medicine depends on understanding how stem cells maintain supportive interactions and adopt particular behaviors. Examining adhesion can help relate cell attachment and signaling to survival, self-renewal, migration, differentiation, and tissue organization. This knowledge supports efforts to study how stem cells behave in regenerative contexts and how their local environment may influence outcomes.
A useful analysis considers which matrix components or neighboring-cell interactions are engaged, how integrins or cadherins form signaling complexes, and how those complexes connect with the cytoskeleton. Researchers can then relate adhesive conditions to polarity, migration, survival, self-renewal, differentiation, and tissue organization. This framework supports biomaterial design aimed at controlling stem cell growth and fate.