Gut Barrier
Gut Barrier - Related Videos
07:18
Oral Gavage in Neonatal Mouse Pups and Functional Assessment of Gut Barrier Integrity Using Ussing Chambers
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2026
This article describes: (1) oral gavage delivery of test molecules to neonatal mice as early as DOL 6; and (2) ex vivo evaluation of colonic epithelial barrier function in biopsies from DOL 10 to weaning (DOL 20) pups by assessing paracellular and transcellular permeability with Ussing chambers.
05:14
Assessment of Gut Barrier Integrity in Mice Using Fluorescein-Isothiocyanate-Labeled Dextran
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Cited by 22 •
2022
In the present study, fluorescein-isothiocyanate-labeled (FITC) dextran is administered to mice via oral gavage to evaluate intestinal permeability both in vivo and in plasma and fecal samples. As gut barrier function is affected in many disease processes, this direct and quantitative assay can be used in diverse research areas.
01:27
Oral Infection Alters the Gut Microbiota in a Rat Model
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2026
Begin with a rat model with sutures tied around its upper molar teeth to support bacterial colonization.Repeatedly smear the sutures with a gum disease-causing oral pathogenic bacterium using a gel-like solution.The bacteria colonize the gum around the sutures.Colonization triggers inflammation and damages the tissues.The bacteria release toxins that diffuse through damaged tissues and enter the bloodstream.These circulating toxins reach the gut, cause inflammation, disrupt the epithelial...
01:15
Assessing the Role of Gut Microbiota in Modulating Hepatitis B Infection in Mice
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2026
Take a control mouse with a normal gut microbiota and an experimental mouse with depleted gut microbiota. The depletion compromises the integrity of the gut barrier.This enables bacteria to translocate through the blood vessel into the liver tissue.Inject both mice with the hepatitis B virus or HBV plasmid to induce an HBV infection in the liver tissue.In the control mouse, the T cell detects an infected liver cell through the interaction of its receptors with viral antigens, and produces...
06:01
Testing the Physiological Barriers to Viral Transmission in Aphids Using Microinjection
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Cited by 7 •
2008
Aphids are effective transmitters of plant viruses. Aphid microinjection of virus, the procedure we will show you today, is a technique allowing researchers to inject virus directly into the hemocoel of the aphid, bypassing the gut, one of the 2 major barriers for virus transmission in a circulative manner. The same technique is also used to inject dsRNA for RNAi.
FAQs
Tight junctions control the movement of substances between neighboring epithelial cells, helping determine which material crosses the lining without passing through the cells themselves. Their regulatory role makes them important for maintaining selective permeability. Changes in junctional control can therefore affect how intestinal contents interact with underlying tissues.
Mucus creates a protective layer over the epithelial surface, while antimicrobial substances help limit contact with potentially harmful microbes and molecules. Together, these components complement the physical control provided by cell junctions. Their action is especially relevant to research on microbial exposure and mucosal protection.
Barrier integrity responds to diet, microbes, inflammation, and injury, so it is not a fixed property of the intestinal lining. These influences can alter the effectiveness of its protective layers and selective passage. Examining them helps researchers connect environmental or biological changes with increased intestinal permeability and mucosal health.
Barrier research can clarify how intestinal microbes and host tissues interact across a selectively controlled interface. It can also help relate changes in permeability to inflammation, infection, or injury. This makes the system useful for connecting cellular barrier behavior with broader biological outcomes in health and disease.
It is relevant when researchers investigate inflammatory disease, infection, nutritional influences, or interventions intended to restore mucosal health. The barrier provides a shared biological context for comparing how these conditions affect protection and permeability, while guiding questions about therapeutic strategies and recovery of barrier function.
Because diet can influence barrier integrity, studies of the gut barrier connect nutrient exposure with epithelial protection and immune defense. This perspective allows biology researchers to examine nutrition alongside microbes, inflammation, and injury rather than treating absorption as an isolated process. It also supports investigation of strategies for maintaining mucosal health.