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JoVE Journal Biology
Testing the Physiological Barriers to Viral Transmission in Aphids Using Microinjection
 

Testing the Physiological Barriers to Viral Transmission in Aphids Using Microinjection

Article doi: 10.3791/700
May 14th, 2008 • Usage Statistics
1, 1, 2
1Plant Pathology, Cornell University, 2Boyce Thompson Institute for Plant Research, Cornell University

Summary
Automatic Translation
May 14th, 2008

Aphids are effective transmitters of plant viruses. Aphid microinjection of virus, the procedure we will show you today, is a technique allowing researchers to inject virus directly into the hemocoel of the aphid, bypassing the gut, one of the 2 major barriers for virus transmission in a circulative manner. The same technique is also used to inject dsRNA for RNAi.

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