Hamilton Syringe
Hamilton Syringe - Related Videos
02:28
Extraction of Apoplastic Bacteria Using a Syringe-Based Pressure Method
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2025
Source: Rufián, J. S., et al, Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue. J. Vis. Exp. (2022)This video demonstrates a syringe-based pressure method for extracting apoplastic bacteria, which proliferate and form microcolonies within the apoplast, the intercellular space between plant cells. The method yields debris-free bacterial samples suitable for downstream single-cell analysis.
09:58
Syringe-injectable Mesh Electronics for Stable Chronic Rodent Electrophysiology
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Cited by 31 •
2018
Mesh electronics probes seamlessly integrate and provide stable, long-term, single-neuron level recording within the brain. This protocol uses mesh electronics for in vivo experiments, involving the fabrication of mesh electronics, loading into needles, stereotaxic injection, input/output interfacing, recording experiments, and histology of tissue containing mesh probes.
05:49
Modeling the Effects of Hemodynamic Stress on Circulating Tumor Cells using a Syringe and Needle
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Cited by 2 •
2021
Here we demonstrate a method to apply fluid shear stress to cancer cells in suspension to model the effects of hemodynamic stress on circulating tumor cells.
09:08
Three-dimensional Printing of Thermoplastic Materials to Create Automated Syringe Pumps with Feedback Control for Microfluidic Applications
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Cited by 3 •
2018
Here we present a protocol to construct a pressure-controlled syringe pump to be used in microfluidic applications. This syringe pump is made from an additively manufactured body, off-the-shelf hardware, and open-source electronics. The resulting system is low-cost, straightforward to build, and delivers well-regulated fluid flow to enable rapid microfluidic research.
11:48
Generation of Induced Pluripotent Stem Cells by Reprogramming Mouse Embryonic Fibroblasts with a Four Transcription Factor, Doxycycline Inducible Lentiviral Transduction System
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Cited by 22 •
2009
The Stemgent Dox Inducible Mouse TF Lentivirus Set can reprogram mouse embryonic fibroblasts (MEFs) to induced pluripotent stem (iPS) cells. Here we demonstrate the protocol for DOX-inducible expression of mouse reprogramming transcription factors Oct4, Sox2, Klf4 and c-Myc to generate iPS colonies that express common mES pluripotency markers.
FAQs
The calibrated barrel provides a defined volume reference, while the closely fitted plunger creates controlled suction during withdrawal and controlled displacement during dispensing. Coordinating these components allows the operator to move small liquid volumes with reduced variation between transfers. This mechanical relationship is central to reproducible sample preparation and quantitative biological or analytical work.
Gas-tight models help limit vapor loss during liquid handling. That feature can preserve the intended sample volume and composition more effectively when evaporation could affect a transfer or measurement. Their use is especially relevant to analytical workflows in which consistent sample delivery supports reliable measurements, including applications associated with chromatography.
Interchangeable and fixed needles support different sample types and delivery tasks. A researcher can select a suitable configuration for the intended biological experiment or analytical transfer, while a fixed needle provides an integrated setup. Matching the needle arrangement to the sample and delivery task helps align the syringe with the practical requirements of the procedure.
Small differences in transferred volume can affect reagent handling, sample preparation, and the consistency of experimental conditions. A precision syringe helps reduce that variation through its calibrated barrel and controlled plunger movement. More reproducible transfers improve comparisons among biological samples and strengthen the reliability of quantitative analysis and subsequent interpretation.
A basic workflow is to select the syringe and needle configuration appropriate for the sample and delivery task, use the plunger to draw the intended liquid volume, and then dispense it in a controlled manner. This sequence supports sample preparation, reagent transfer, and other procedures where accurate, repeatable small-volume handling is required.
Common applications include biological sample preparation, microinjection, chromatography, and accurate reagent handling. In microinjection, controlled delivery supports work with very small biological targets, while chromatography uses precise sample transfer for analytical measurements. Across these settings, the instrument contributes to consistent volumes, quantitative analysis, and improved experimental reproducibility.