Hek-293 Cell Transfection
Hek-293 Cell Transfection - Related Videos
12:59
Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells
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Cited by 23 •
2011
Reliable method for highly efficient in vitro expression and subsequent electrophysiological recording of recombinant voltage-gated ion channels in cultured human embryonic kidney cells (HEK-293T).
02:22
Production of Pseudovirus Using Plasmid Transfection in Human Cells
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2026
Source: Chang, X., et al. Preparation of Pseudo-Typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity. J. Vis. Exp. (2021)This video demonstrates the production of influenza pseudoviruses in human cells using calcium phosphate-mediated plasmid transfection, enabling safe generation of single-cycle viral particles for downstream applications such as antibody neutralization assays under biosafety level 2 conditions.
11:42
High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension
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Cited by 76 •
2015
Laboratory-scale production of eukaryotic proteins with appropriate post-translational modification represents a significant barrier. Here is a robust protocol with rapid establishment and turnaround for protein expression using a mammalian expression system. This system supports selective amino acid, selective labeling of proteins and small molecule modulators of glycan composition.
09:33
Rapid Isolation of the Mitoribosome from HEK Cells
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Cited by 25 •
2018
Mitochondria have specialized ribosomes that diverged from their bacterial and cytoplasmic counterparts. Here we show how mitoribosomes can be obtained from their native compartment in HEK cells. The method involves isolation of mitochondria from suspension cells and consequent purification of mitoribosomes.
10:24
Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells
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Cited by 26 •
2011
Despite recent advancements in genetic modification, transfection of human embryonic stem cells (HESCs) remains a capricious process. To our knowledge, systematic and efficient methods to transfect human induced pluripotent stem cells (iPSCs) have not been reported. Here, we describe robust protocols to efficiently transfect and nucleofect human iPSCs.
FAQs
These approaches are alternative ways to move nucleic acids across the cell membrane. Lipid-based reagents, calcium phosphate, and electroporation are all identified as transfection options, but the overview does not rank them or assign a universal efficiency advantage. The appropriate choice therefore depends on the experiment’s delivery needs and the downstream assay.
Transient expression produces a temporary change in gene expression, while stable maintenance preserves introduced genetic material under appropriate selection conditions. This distinction determines the experimental timescale and the type of result researchers can obtain. Temporary expression suits short-term analyses, whereas stable maintenance supports studies requiring continued genetic activity or longer-term evaluation.
Selection conditions matter when the goal extends beyond short-term expression. Under appropriate selection conditions, introduced genetic material can be maintained more stably in HEK-293 cells, whereas transient expression is used when researchers primarily need a temporary change in gene expression. This distinction affects how experiments are designed and interpreted over time.
A general workflow begins by choosing DNA or RNA according to the intended gene-expression experiment, followed by selecting a delivery approach such as a lipid-based reagent, calcium phosphate, or electroporation. Researchers then assess the resulting expression or use the modified cells in an appropriate assay, with selection added when more stable maintenance is required.
Introducing suitable nucleic acids into HEK-293 cells can direct expression of recombinant proteins, making the system useful in biotechnology and molecular biology. Researchers can use either transient expression for short-term production or stable maintenance when appropriate selection conditions support continued genetic activity. The resulting cells provide a platform for evaluating protein production in experimental studies.
The system supports several functional readouts, including altered gene expression, gene-function testing, signaling-pathway studies, reporter-construct evaluation, and assessment of therapeutic candidates. These applications allow researchers to connect introduced nucleic acids with measurable biological responses. The selected assay determines whether the experiment emphasizes regulation, signaling, candidate activity, or expression behavior.
HEK-293 cell transfection combines adaptable nucleic-acid delivery with compatibility across diverse assays. That combination supports research in biotechnology, virology, pharmacology, and gene regulation, in addition to molecular and cellular biology. Its value comes from allowing researchers to modify gene expression and then examine protein production, signaling, reporter activity, or therapeutic responses in a common experimental system.