High-throughput Yeast Array
High-throughput Yeast Array - Related Videos
08:57
High-throughput Yeast Plasmid Overexpression Screen
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Cited by 12 •
2011
Here we describe a plasmid overexpression screen in Saccharomyces cerevisiae, using an arrayed plasmid library and a high-throughput yeast transformation protocol with a liquid handling robot.
10:02
Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast
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Cited by 18 •
2016
Accurate quantification of vesicular trafficking events often provides key insights into roles for specific proteins and the effects of mutations. This paper presents methods for using superecliptic pHluorin, a pH-sensitive GFP variant, as a tool for quantification of endocytic events in living cells using quantitative fluorescence microscopy and flow cytometry.
08:30
Yeast Maintenance
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2023
Research performed in the yeast Saccharomyces cerevisiae has significantly improved our understanding of important cellular phenomona such as regulation of the cell cycle, aging, and cell death. The many benefits of working with S. cerevisiae include the facts that they are inexpensive to grow in the lab and that many ready-to-use strains are now commercially available. Nevertheless, proper maintenance of this organism is critical for successful experiments. This video will provide an overview...
07:48
Yeast Reproduction
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2023
Saccharomyces cerevisiae is a species of yeast that is an extremely valuable model organism. Importantly, S. cerevisiae is a unicellular eukaryote that undergoes many of the same biological processes as humans. This video provides an introduction to the yeast cell cycle, and explains how S. cerevisiae reproduces both asexually and sexually Yeast reproduce asexually through a process known as budding. In contrast, yeast sometimes participate in sexual reproduction, which is important because...
06:49
Isolating Nucleic Acids from Yeast
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2023
One of the many advantages to using yeast as a model system is that large quantities of biomacromolecules, including nucleic acids (DNA and RNA), can be purified from the cultured cells. This video will address the steps required to carry out nucleic acid extraction. We will begin by briefly outlining the growth and harvest, and lysis of yeast cells, which are the initial steps common to the isolation of all biomacromolecules. Next, we will discuss two unique purification methods for the...
FAQs
Their organized layout places many yeast strains in a format that allows the same environmental or chemical condition to be assessed across different genetic backgrounds. Comparing growth, survival, or another measured phenotype helps reveal strain-specific responses. Those response patterns can then connect differences in phenotype with particular genes, supporting genome-wide genetic screens and gene-function studies.
Robotic pinning transfers small amounts of each yeast strain or colony onto defined media in a controlled, repeatable pattern. This automated handling makes it practical to test many strains in parallel while maintaining consistent placement and treatment conditions. The resulting organization supports reproducible comparisons of growth, survival, or other phenotypes across the array.
Defined media and chemical treatments establish the conditions under which yeast phenotypes are compared. Changing those conditions can expose differences in growth or survival among strains, helping researchers associate environmental or chemical responses with genetic backgrounds. This approach supports drug sensitivity testing and investigations of cellular pathways by showing which strains respond differently under a selected condition.
A phenotype pattern becomes informative when responses are compared across strains with different genetic backgrounds. If particular strains show altered growth, survival, or treatment sensitivity, the associated genetic differences provide clues about gene function. Linking these observations to genes allows the array to support genome-wide screens and helps identify genes involved in cellular pathways.
A typical workflow begins by organizing yeast strains or colonies, then using robotic pinning or automated handling to place small amounts onto defined media. Researchers expose the arranged strains to environmental or chemical conditions and measure growth, survival, or another phenotype. They compare the resulting responses across strains to connect observed differences with genetic backgrounds or genes.
These arrays are useful when researchers need to examine many genetic backgrounds or conditions efficiently. Applications include genome-wide genetic screens, gene-function studies, drug sensitivity testing, and analysis of cellular pathways. Because yeast responses can help identify conserved biological mechanisms and candidate targets for further study, the approach connects large-scale screening with broader questions in biology.