Hindbrain Injection Site
Hindbrain Injection Site - Related Videos
09:17
Labeling and Imaging Cells in the Zebrafish Hindbrain
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Cited by 7 •
2010
Key to understanding the morphogenetic processes that shape the early embryo is the ability to image cells at high resolution. We describe here a technique for labeling single cells or small clusters of cells in whole zebrafish embryos with membrane-targeted Green Fluorescent Protein.
10:57
Mouse Hindbrain Ex Vivo Culture to Study Facial Branchiomotor Neuron Migration
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Cited by 4 •
2014
Embryonic neurons are born in the ventricular zone of the neural tube, but migrate to reach appropriate targets. Facial branchiomotor (FBM) neurons are a useful model to study neuronal migration. This protocol describes the wholemount ex vivo culture of mouse embryo hindbrains to investigate mechanisms that regulate FBM migration.
10:04
Electroporation of the Hindbrain to Trace Axonal Trajectories and Synaptic Targets in the Chick Embryo
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Cited by 9 •
2013
How neuronal networks are established in the embryonic brain is a fundamental question in developmental neurobiology. Here we combined an electroporation technique with novel genetic tools, such as Cre/Lox–plasmids and PiggyBac-mediated DNA transposition system in the avian hindbrain to label dorsal interneurons and track their axonal projections and synaptic targets at various developmental stages.
11:39
The Mouse Hindbrain As a Model for Studying Embryonic Neurogenesis
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Cited by 2 •
2018
This article demonstrates how the mouse embryonic hindbrain can be used as a model for studying developmental neurogenesis in both whole organ and tissue section preparations.
03:45
Intraductal Injection: Delivering Injection Mix into the Ducts of the Mouse Mammary Gland
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2023
This video describes an intraductal injection technique where we deliver injection mix into the ducts of mouse mammary glands.
FAQs
Placement determines which neural circuits receive local exposure to the administered substance or tracer. Consequently, even within the hindbrain, selecting a different anatomical location can change the circuit-level response being examined. Precise targeting helps researchers relate subsequent changes in neural activity or behavior to the function of the exposed region.
The hindbrain contributes to autonomic, sensory, and motor functions, so an imprecise target may expose a different functional circuit than intended. Accurate placement strengthens the connection between the selected site and the observed outcome. This is especially important when interpreting effects on brain-body regulation, neural activity, or behavior.
The site can receive experimental compounds or tracers, according to the purpose of the study. Compounds allow researchers to examine localized pharmacological effects, whereas tracers help investigate neural circuitry. In both cases, delivery to the selected location supports analysis of how a defined hindbrain region relates to biological responses.
A study can assess changes in neural activity and behavior after delivering a substance or tracer to a selected hindbrain location. Comparing these outcomes helps connect local exposure with both circuit-level and observable effects. This combined perspective is useful for examining how hindbrain regions participate in coordinated biological functions.
The workflow consists of guiding a needle or cannula to the selected hindbrain location, administering the experimental compound or tracer, and assessing resulting changes in neural activity or behavior. The central procedural requirement is accurate targeting, because the chosen location determines which neural circuits receive local exposure and how results are interpreted.
Researchers use a targeted site when they need to associate an experimental substance or tracer with a particular hindbrain location and its connected circuits. This approach supports focused studies of brain-body regulation, neural circuitry, pharmacological effects, and disease mechanisms, rather than treating the entire hindbrain as a single functionally uniform region.
By delivering an experimental compound or tracer to a defined hindbrain location and examining neural or behavioral changes, researchers can evaluate how particular circuits participate in disease-related processes. The approach links regional exposure with functional outcomes, providing biological context for investigating altered brain-body regulation and other mechanisms relevant to disease.