Inositol Triphosphate
Inositol Triphosphate - Related Videos
15:22
Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
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Cited by 4 •
2014
The protocol described herein aims to explain and abridge the numerous obstacles in the way of the intricate route leading to modified nucleoside triphosphates. Consequently, this protocol facilitates both the synthesis of these activated building-blocks and their availability for practical applications.
10:34
Preparation of Quality Inositol Pyrophosphates
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Cited by 27 •
2011
Inositol pyrophosphates play an important role in human pathologies such cancer, diabetes and obesity; however, the exact mechanism of action is a matter of dispute. The lack of commercially available inositol pyrophosphates renders detailed studies problematic. Here we describe a simple protocol to produce and isolate milligrams of inositol pyrophosphates.
09:22
Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry
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Cited by 37 •
2021
A procedure for capillary electrophoresis electrospray ionization mass spectrometry for the absolute quantitation of inositol pyrophosphates from mammalian cell extracts is described.
09:01
Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC
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Cited by 8 •
2020
Here we describe strong anion exchange high-performance liquid chromatography of [3H]-myo-inositol-labeled seedlings which is a highly sensitive method to detect and quantify inositol polyphosphates in plants.
03:43
An Imaging Technique to Study the Calcium Response in Bacteria-Infected Cells
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2025
This video demonstrates a technique for detecting local and global calcium ion response upon Shigella infection of human epithelial cells. Shigella invades host cells and releases invasion effectors, impacting intracellular calcium levels. The amplitude and frequency of the local and global cytosolic calcium are studied using a fluorescent indicator.
FAQs
An activated cell-surface receptor stimulates phospholipase C, which acts on phosphatidylinositol 4,5-bisphosphate in the plasma membrane. This cleavage produces inositol triphosphate and initiates the messenger pathway. The sequence links an extracellular signal to a defined enzymatic event at the membrane, allowing receptor activity to influence calcium release inside the cell.
IP3 is soluble, so it can diffuse through the cytoplasm after its production at the plasma membrane. It reaches gated receptors on the endoplasmic reticulum and binds them, promoting the release of stored calcium ions. This mobility connects two separate cellular compartments and enables receptor stimulation to produce an intracellular calcium signal.
IP3-gated receptors on the endoplasmic reticulum serve as the intracellular targets that convert messenger binding into calcium release. Their activity determines whether calcium stored in this compartment enters the cytoplasm. That release provides the signal through which receptor activation can influence downstream processes, including muscle contraction, secretion, metabolism, and gene expression.
The pathway begins with an event in the plasma membrane but carries its message inward through a soluble messenger. Rather than limiting the response to the receptor’s immediate location, IP3 communicates with calcium-release machinery on the endoplasmic reticulum. This compartment-to-compartment organization allows surface-receptor activity to regulate intracellular calcium-dependent responses.
A useful conceptual sequence starts with an activated cell-surface receptor, followed by phospholipase C stimulation and cleavage of phosphatidylinositol 4,5-bisphosphate. The analysis then follows IP3 movement through the cytoplasm, binding to endoplasmic-reticulum receptors, calcium release, and the resulting cellular response. Tracking these steps helps identify where signaling is initiated and how it produces an outcome.
Studies of inositol triphosphate signaling can connect receptor activity with calcium-dependent changes in cell behavior. Relevant outcomes include muscle contraction, secretion, metabolic regulation, and altered gene expression. The pathway is therefore useful in biology research focused on cell communication and physiological responses, particularly when investigators need to relate an external signal to a defined intracellular effect.