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Chapters
- 00:05Title
- 01:22Enzymatic Reaction
- 02:22Polyacrylamide Gel Casting and Loading
- 03:54Isolation of IP7
- 07:26Determination of IP7 Concentration and Purity
- 08:24Representative Quality Inositol Pyrophosphates
- 10:08Conclusion
Inositol pyrophosphates play an important role in human pathologies such cancer, diabetes and obesity; however, the exact mechanism of action is a matter of dispute. The lack of commercially available inositol pyrophosphates renders detailed studies problematic. Here we describe a simple protocol to produce and isolate milligrams of inositol pyrophosphates.
