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Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC

Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC

DOI:
10.3791/61495-v

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09:01 min

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June 26, 2020

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, , ,
1Department of Plant Nutrition, Institute of Crop Science and Resource Conservation,University of Bonn

Chapters

  • 00:04Introduction
  • 00:50HPLC System Set Up
  • 01:37Plant Cultivation and Labeling with [3H]-myo-inositol
  • 03:02Extraction of Soluble InsPs
  • 04:31HPLC
  • 05:51Data Analysis
  • 06:45Results: InsP profiles of A. thaliana and L. japonicus
  • 08:15Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe strong anion exchange high-performance liquid chromatography of [3H]-myo-inositol-labeled seedlings which is a highly sensitive method to detect and quantify inositol polyphosphates in plants.

Tags

Keywords: Inositol PolyphosphatesRadiolabelingSAX-HPLCPlant MetabolismPlant DevelopmentExtractionQuantificationLotus SeedsMyo-inositolSample PreparationHPLC Setup

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