Intestinal Cell Line
Intestinal Cell Line - Related Videos
10:58
A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids
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Cited by 81 •
2015
In this video protocol we give a step by step explanation of lentiviral transduction in organoids of primary intestinal epithelium and of processing and downstream analysis of these cultures by quantitative RT-PCR, RNA-microarray and immunohistochemistry.
09:25
Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine
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Cited by 68 •
2012
Here, we detail a methodology for the rapid isolation of mouse intestinal dendritic cells (DCs) and macrophages. Phenotypic characterization of intestinal DCs and macrophages is performed using multi-color flow cytometric analysis while magnetic bead enrichment followed by cell sorting is used to yield highly pure populations for functional studies.
05:34
Oral Delivery of Transformed Yeast Cells into the Mouse Intestinal Immune System
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2025
This video showcases the oral administration of transformed yeast cells into a mouse model. The transformed yeast cells are orally administered using a gavage technique, reaching the mouse's stomach. Later, the cells migrate to the small intestine, where microfold cells engulf them and facilitate their transfer to Peyer's patches and gain access to the intestinal immune system.
07:48
Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models
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Cited by 3 •
2015
Intestinal epithelial stem cells (ISCs) are intermingled with Paneth cells. These cells are differentiated progeny of the ISC, which support the ISCs and provide antibacterial protection. Here we demonstrate how we used transgenic conditional mouse models to establish that Paneth cells play a crucial role in maintaining the intestinal epithelia.
07:00
Using Caco-2 Cells to Study Lipid Transport by the Intestine
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Cited by 11 •
2015
Caco-2 cells can serve as an in vitro model to study the enterocyte transport of lipids, and lipid-soluble drugs/vitamins. The permeable membrane system separates the apical from the basolateral compartment, while the lentivirus expression system offers an effective gene overexpression method. The isolation of lipoproteins is confirmed by TEM.
FAQs
Culture conditions can determine whether cells mainly proliferate or acquire specialized epithelial phenotypes. They also affect formation of intercellular junctions and development of barrier and transport properties. Consequently, investigators select and control conditions according to the endpoint, such as modeling epithelial integrity, nutrient absorption, or drug permeability, rather than treating every intestinal cell line as biologically equivalent.
Intercellular junctions help cultured intestinal cells reproduce coordinated epithelial behavior rather than acting as isolated cells. Their formation is associated with barrier properties that can be examined experimentally, while transport characteristics support studies of movement across the epithelial layer. These features make junction development relevant when evaluating intestinal responses, permeability, or tissue-like organization.
Intestinal cell lines provide reproducible, controlled systems for examining selected aspects of intestinal structure and function, while animal models represent more complex biological settings. Using both approaches can help researchers investigate epithelial responses and evaluate therapeutic compounds before or alongside broader biological studies. This complementary role also supports refinement of experimental strategies and tissue-engineering approaches.
A useful maintenance strategy considers whether the cells remain proliferative or develop specialized epithelial characteristics under controlled laboratory conditions. Researchers can also examine intercellular junctions, barrier behavior, and transport properties to determine whether the culture reflects the intended intestinal function. Monitoring these features helps align the cultured system with studies of absorption, permeability, inflammation, or disease.
These cultures are useful when researchers need to investigate nutrient absorption, drug permeability, host-microbe interactions, inflammation, or intestinal disease in a controlled experimental setting. Their reproducibility allows specific epithelial responses to be examined without relying solely on animal studies. The same systems can support evaluation of therapeutic compounds and comparison of responses under different culture conditions.
For drug research, cultured intestinal cells can provide models for examining permeability and epithelial responses to therapeutic compounds. In tissue engineering, their ability under suitable conditions to form specialized phenotypes, intercellular junctions, and barrier properties offers a biological reference for developing more tissue-like intestinal systems. These applications connect controlled cell studies with efforts to model intestinal function.