Intracellular Recording
Intracellular Recording - Related Videos
02:36
In Vivo Recording of Intracellular Signals from a Single Neuron
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2025
This video demonstrates the recording of electrical signals from single brain neurons using a fine-tipped glass micropipette and a specialized setup. The micropipette is carefully inserted into the rat's brain and is buzzed to penetrate the neuron to record internal neuronal electrical signals.
11:53
Intracellular Recording, Sensory Field Mapping, and Culturing Identified Neurons in the Leech, Hirudo medicinalis
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Cited by 14 •
2013
This article describes three nervous system preparations using leeches: intracellular recording from neurons in ventral ganglia, culturing neurons from ventral ganglia, and recording from a patch of innervated skin to map sensory fields.
08:33
Determination of Photoreceptor Cell Spectral Sensitivity in an Insect Model from In Vivo Intracellular Recordings
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Cited by 14 •
2016
The electrophysiological technique of intracellular recording is demonstrated and used to determine spectral sensitivities of single photoreceptor cells in the compound eye of a butterfly.
03:48
In Vivo Intracellular Recording of Photoreceptor Cell Spectral Sensitivity in a Butterfly
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2025
This video demonstrates the procedure for in vivo intracellular recording of photoreceptor cells in the eye of a butterfly and determining the spectral sensitivity of individual cell types. This technique enables the study of sensitivities of individual photoreceptor cells to different light stimuli.
11:42
Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo
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Cited by 14 •
2016
Sharp microelectrodes enable accurate electrophysiological characterization of photoreceptor and visual interneuron output in living Drosophila. Here we show how to use this method to record high-quality voltage responses of individual cells to controlled light stimulation. This method is ideal for studying neural information processing in insect compound eyes.
FAQs
Controlled current injection allows researchers to perturb a living cell while measuring its electrical response. The resulting changes can show how the cell responds to imposed signals, complementing measurements made under unaltered conditions. This capability is particularly useful for characterizing electrical responsiveness alongside naturally occurring membrane potential changes, action potentials, or synaptic responses.
Membrane-potential measurements track the electrical state across the cell membrane, whereas ion-current measurements track electrical flow associated with ion-channel activity. These readouts provide complementary information: voltage recordings can characterize resting potential, action potentials, and synaptic responses, while current recordings help examine ion-channel behavior and cellular effects caused by drugs or genetic changes.
Access to the cytoplasm lets the recording apparatus detect electrical events from within the cell rather than only observing signals outside it. This connection supports direct monitoring of membrane potential and ion currents, as well as controlled current injection. Consequently, researchers can relate cellular electrical changes to signaling processes such as neuronal communication and muscle contraction.
The approach uses either a fine glass microelectrode or a patch-clamp pipette to establish the electrical connection with a living cell. The cell must remain under defined conditions during measurement so its electrical behavior can be interpreted consistently. This setup supports recording membrane potential, monitoring ion currents, or applying controlled current without removing the cell from the experiment.
A typical workflow maintains a living cell under defined conditions, establishes an electrical connection through a fine glass microelectrode or patch-clamp pipette, and then selects the desired measurement. Researchers may monitor membrane potential, record ion currents, or inject controlled current. The resulting traces can be examined for resting potential, action potentials, synaptic responses, or ion-channel activity.
Biologists apply this technique to study neuronal communication, muscle contraction, and sensory processing, as well as cellular responses to drugs or genetic changes. The measurements can reveal resting potential, action potentials, synaptic responses, and ion-channel activity. These outcomes connect electrical behavior with broader biological functions and help characterize how altered conditions affect living cells.