Ischemic Cuff Construction
Ischemic Cuff Construction - Related Videos
13:19
Mouse Model for Pancreas Transplantation Using a Modified Cuff Technique
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Cited by 6 •
2017
Among abdominal solid organ transplantation, pancreatic grafts are prone to develop severe ischemia reperfusion injury-associated graft damage, leading eventually to early graft loss. This protocol describes a model of murine pancreas transplantation using a non-suture cuff technique, ideally suited for analyzing these early, deleterious damages.
10:30
A Piglet Model of Neonatal Hypoxic-Ischemic Encephalopathy
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Cited by 37 •
2015
Hypoxic-ischemic encephalopathy following perinatal asphyxia can be studied using animal models. We demonstrate the procedures necessary for establishing a piglet model of neonatal hypoxic-ischemic encephalopathy.
07:51
Implantation of a Carotid Cuff for Triggering Shear-stress Induced Atherosclerosis in Mice
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Cited by 30 •
2012
The constricting cuff presented in this article is designed to induce atherosclerosis in the murine common carotid artery. Due to the conical shape of its inner lumen the implanted cuff generates well-defined regions of low, high and oscillatory shear stress triggering the development of atherosclerotic lesions of different inflammatory phenotypes.
05:25
Knotless Independent Double-Row Repair and Biceps Augmentation for Anterosuperior Rotator Cuff Tears
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2026
This is a technical note describing a cost-efficient, knotless, independent, double-row rotator cuff repair for medium to large anterosuperior rotator cuff tears with simultaneous biceps augmentation and subscapularis repair.
04:20
Non-Invasive Limb Ischemic Preconditioning in a Rat Model for Neuroprotection
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2025
Source: Brandli, A. Remote Limb Ischemic Preconditioning: A Neuroprotective Technique in Rodents. J. Vis. Exp. (2015)This video demonstrates a non-invasive limb ischemic preconditioning protocol in rats to induce systemic neuroprotection. The procedure involves controlled arterial occlusion and reperfusion, triggering cellular stress pathways that release protective factors. These factors enhance resistance to ischemic and metabolic injuries in distant organs such as the brain and retina.
FAQs
Cuff dimensions help determine how pressure is distributed across the targeted vessel or limb. A design that matches the intended tissue region supports more consistent restriction, whereas unsuitable dimensions may produce uneven exposure or affect a different region than planned. Controlling this variable allows researchers to compare tissue responses across experiments with greater confidence.
Pressure determines the degree to which perfusion and oxygen delivery are reduced, while application time controls how long the tissue experiences that reduction. Changing either variable can alter ischemia severity and duration. Keeping both conditions defined and consistent is therefore essential when investigating injury, vascular regulation, or differences in tissue resilience.
A temporary restriction creates a defined period of oxygen deprivation that can be followed by restoration of blood flow. This sequence allows investigators to examine both the tissue response during ischemia and the changes that occur during recovery. The approach is useful for distinguishing effects associated with reduced perfusion from responses linked to restored circulation.
A basic workflow begins by defining the tissue region and selecting cuff dimensions appropriate for that target. The device is then assembled, positioned around the relevant vessel or limb, and applied with specified pressure for a defined period. Recording placement, pressure, dimensions, and timing improves standardization and makes results easier to interpret.
Researchers may select this approach when they need a controlled, localized model of reduced blood flow rather than an uncontrolled injury. It supports studies of oxygen deprivation, vascular regulation, tissue damage, and recovery after circulation returns. The controlled exposure also makes it useful for evaluating therapeutic interventions and comparing how tissues tolerate ischemic stress.
Results can reveal how a defined tissue region responds to reduced oxygen delivery, how vascular regulation changes during the exposure, and how effectively the tissue recovers after flow is restored. Comparing responses under standardized cuff conditions helps identify differences in injury sensitivity and recovery capacity, providing biological context for ischemic disease and intervention studies.