Mouse Model for Pancreas Transplantation Using a Modified Cuff Technique

Benno Cardini; Rupert Oberhuber; Sven R Hein; Rebecca Eiter; Martin Hermann; Markus Kofler; Stefan Schneeberger; Gerald Brandacher; Manuel Maglione

doi:10.3791/54998

Journal

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Medicine

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Mouse Model for Pancreas Transplantation Using a Modified Cuff Technique

JoVE Journal
Medicine
Author Produced

This content is Free Access.

JoVE Journal Medicine

Mouse Model for Pancreas Transplantation Using a Modified Cuff Technique

10,942 Views

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13:19 min

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December 16, 2017

DOI:

10.3791/54998-v

DOI:

10.3791/54998-v

•

13:19 min

December 16, 2017

•

10926 Views

Benno Cardini*¹, Rupert Oberhuber*¹, Sven R Hein¹, Rebecca Eiter¹, Martin Hermann², Markus Kofler^1,3, Stefan Schneeberger¹, Gerald Brandacher^1,4, Manuel Maglione¹

¹Center of Operative Medicine, Department of Visceral, Transplant and Thoracic Surgery,Medical University Innsbruck ²Department of Anesthesiology and Critical Care Medicine,Medical University Innsbruck ³Department of Cardiac Surgery,Medical University Innsbruck ⁴Department of Plastic and Reconstructive Surgery, Vascularized Composite Allotransplantation (VCA) Laboratory,Johns Hopkins University School of Medicine

Summary

Among abdominal solid organ transplantation, pancreatic grafts are prone to develop severe ischemia reperfusion injury-associated graft damage, leading eventually to early graft loss. This protocol describes a model of murine pancreas transplantation using a non-suture cuff technique, ideally suited for analyzing these early, deleterious damages.